Chemicon (now Millipore)
Mouse anti-GAD67
MAB5406
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Browse Similar Products: Anti-GAD67 Immunohistochemistry Antibodies
In these experiments, I wanted to investigate whether a novel receptor (for olfactory bulb tissue) was co-expressed with known subpopulations. The antibodies used target known histological markers for these subpopulations. Here, I wanted to determine GAD67 location in fixed brain tissue. This antibody had been left in the refrigerator for years before I used it. It worked well but labeled only the processes, not the cell bodies, of GAD67-positive cells.
Immunohistochemistry
PFA-fixed brain tissue slice
1:10,000 for 48 hours at 4 degrees C
1% normal horse serum in PBS (0.4% Triton-X 100)
1:1000 Cy3-conjugated horse anti-mouse for 2 hours at room temperature
NA
Cy3 fluorescence via fluorescent confocal microscopy
Cell processes were bright and easily identified. Staining occurred throughout the slice, as GAD67 is known to be more prominent than GAD65. Staining did not occur in lamina known to not express GAD65 or GAD67.
Can be used at a much more dilute concentration than the company states.
Works for a long time at a good dilution.
Takes a bit of fine-tuning to get the dilutions correct.
A good antibody for IHC but not for immunoblot. Recommended.