EMD Millipore
Rabbit anti-fibrinogen
341552
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Browse Similar Products: Fibrinogen Western blot Antibodies
This is a cheap primary antibody compared with all other common primary antibodies from rabbit. Dilution is also okay, 1:2000 does the job. It works quite well against pure bovine fibrinogen as I tried (see image), while a-chain is not well recognized, b and r chain are fine. However, if your sample is from a tissue, blood or organ, this antibody does not work at all.
Western Blot
Pure bovine fibrinogen
1:2000, 1 hour in 5% milk TBST
5% milk TBST
1:2000, 1 hour in TBST
NA
ECL
A pure protein on the western blots works well with this antibody, although the a-chain is not so well recognized. However, the bands are clean and readable.
None
Cheap price, works OK
Does not work with tissue homogenate
Depend on the experiment you do. Works with pure protein.