Chemicon (now Millipore)
Goat anti-calretinin
AB-1550
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I wanted to detect calretinin in fixed brain tissue via immunohistochemistry. I pulled this antibody out of a -20 degrees C freezer and tried to use it. It works very well while highly dilute and gives great pictures.
Immunohistochemistry
PFA-fixed brain tissue slice
1:30,000 for 48 hours at 4 degrees C
1% normal donkey serum in PBS (0.1% Triton-X 100)
1:1000 Cy3-conjugated donkey anti-goat for 2 hours at room temperature
NA
Cy3 fluorescence via fluorescent confocal microscopy
Filled cells but images were crisp and clear. Antibody continues to work after being left for years in the -20 degree freezer.
Did not show up for Ni-DAB stain with ABC kit until 1:100,000 dilution.
Works at low dilution, works well.
May be due to lot, rather than antibody overall.
Great antibody, highly recommended for IHC. Worked well for Ni-DAB staining and fluorescence.