EMD Millipore
Anti-phospho-Histone H2A.X (Ser139)
05-636
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H2AX is a histone variant that is required for DNA damage response. Phosphorylation of H2AX on S139 can be induced by ionizing radiation and genotoxic stresses. Here I tested the camptothecin (CPT)-induced H2AX pS139 in cancer cells.
Immunofluorescence
U2OS cells
Dilution 1:500 in PBS/0.05% Tween20/3% BSA blocking buffer. Overnight incubation at 4C
3% BSA / 0.05% Tween20 in PBS
Alexa488--conjugated anti-mabbit 2nd antibody at dilution of 1:250 and 1 hour of incubation at room temperature
NA
Fluorescent microscope
The 05-636 antibody worked very nicely for IF. The phosphorylated H2AX S139 foci were evident in CPT treated cells, but not in undamaged cells.
None
Super specific and sensitive
People who wants to work on phospho-H2AX S139 should definitely try this antibody.