EMD Millipore
Anti-microtubule associated protein 2 (MAP2)
AB5622
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Following MPTP, we wanted to quantify the total number of surviving neurons in the SNpc using an antibody specific for MAP2. Fixed brain tissue was sectioned through the midbrain containing the SN and stained (free floating) with anti-MAP2 and nuclei stained with DAPI containing medium. Stereological analysis was performed to quantify neuron numbers.
Immunofluorescence
Fixed brain tissue
1:1000 in 2% normal donkey serum/TBS
5% NDS/TBS
1:2000 in 2% NDS/TBS, 1 hour, room temp
NA
Texas Red fluroescence
We were able to distinguish cell bodies from other staining but the images weren't fantastic (there was a lot of other staining in the tissues). But with the addition of the DAPI-containing medium we could pick out a cell confidently.
None
Easy to optimize concnetrations
Doesn't result in publication-quality images
For what we use it for, this antibody works well enough that we will continue to order it.