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Date Published
Antibody for Detecting Apoptotic Cells
(1)
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February 15, 2018
We used the Annexin V antibody to detect cytotoxicity of CAR-T cells on Raji cell line, which is a lymhoblastoid cell with B cell ...
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Antibody Is Bright And Stains CD62L+ T Cells Well
(1)
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BioLegend
February 15, 2018
One of the projects in our lab is studying the role and function of T cells in an active influenza A viral infection. In order to do that, ...
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Great Flow Antibody To Identify Memory Pre-Cursors Based On IL-7 Receptor Expression
(1)
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Thermo Fisher Scientific
February 15, 2018
We are an immunology lab studying CD8 T cell memory differentiation. CD127 is a hallmark memory precursor marker. during an acute immune ...
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Human CD27 APC Antibody
(1)
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BD Biosciences
February 14, 2018
Stimulation of T cells induces activation and proliferation. Successful stimulation requires signaling through co-stimulatory receptors, ...
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Decent CD4 Antibody Staining
(1)
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Thermo Fisher Scientific
February 14, 2018
This antibody titrates out well but not as well as other fluorophores of the same clone. It can be used in a variety of applications but may...
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PCR Master Mix
(1)
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Quanta BioSciences
February 14, 2018
I am working in the field of environmental microbiology and my objectives include development of molecular methods for the detection of ...
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CD11b for BMDC
(1)
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BioLegend
February 14, 2018
This antibody was used to stain BMDC for CD11b and other surface molecules to make sure they differentiated correctly in culture during a ...
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beta-Actin Antibody for Western Blot
(1)
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Abcam
February 13, 2018
This antibody was purchased as a control to normalize protein expression to. It was selected because we multiplex this with several mouse ...
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Alexa Fluor 488 Anti-Human CD20
(1)
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BioLegend
February 13, 2018
500,000 cells (Daudi, Ramos, MY5) were stained with 1:100 (red histograms) 302316 in PBS+0.5% BSA for 1 hour at 4oC, washed once in 1X PBS ...
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Excellent Antibody
(1)
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Santa Cruz Biotechnology, Inc.
February 13, 2018
Proliferating cell- Nuclear antigen is a 30 kDa protein that appears in early G and S phase of the cell cycle. It functions in DNA ...
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IgG2b Kappa Isotype Control
(1)
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Thermo Fisher Scientific
February 13, 2018
Microglial cells from the brains of 1-3 day old pups were isolated and cultured for 7 days in the presence of M-CSF to enrich for microglia....
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Purity Check Of Primary Microglial Cells Utilizing The CD45 Monoclonal Antibody
(1)
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Thermo Fisher Scientific
February 12, 2018
Primary microglial cells were isolated from the brains of 1-3 day old pups after 7 days in culture with M-CSF. The purity of the microglial ...
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EIF2-Alpha Antibody For Western Blot
(1)
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Santa Cruz Biotechnology, Inc.
February 12, 2018
This antibody was selected as a control to normalize the ratio of phosphorylated eIF2a to total eIF2-alpha. The context of this application...
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CD30 Antibody
(1)
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BioLegend
February 12, 2018
This antibody has been used to detect human CD30 by FACS on lymphoma cell lines
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Good CD28 Staining On Human PBMCs
(1)
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BD Biosciences
February 12, 2018
The aim of this study was to observe CD28 downregulation on CD4+ T cells infected with HIV encoding different Nef alleles. I chose this ...
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Good Antibody For Staining CD24+ Cells
(1)
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BD Biosciences
February 09, 2018
This antibody was used to identify CD24+ cells among CD11c+ cells in mouse lungs.
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Excellent Flow Cytometry Antibody For Mouse CD4
(1)
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Thermo Fisher Scientific
February 09, 2018
I used this antibody to identify Tregs in spleen, thymus and peripheral blood based on CD4+CD25+Foxp3+ expression pattern. This antibody ...
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Great Antibody To Identify Terminal Effector CD8 T Cells
(1)
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Thermo Fisher Scientific
February 09, 2018
We are an immunology lab studying CD8 T cell memory differentiation. KLRG1 is a hallmark marker to identify terminal effector T cells. We ...
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CHOP (DDIT3) Antibody
(1)
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Abcam
February 09, 2018
This antibody was used to detect the presence of endoplasmic reticulum stress / unfolded protein response activation, specifically the IRE1 ...
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Human CD57 APC for FACS
(1)
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BD Biosciences
February 08, 2018
Human T cells maintain lifelong immunity to pathogens. However certain infections induce defective immune responses and involve CD8+ T cells...
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The Antibody Stains BrdU+ Cells Very Well
(1)
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BioLegend
February 08, 2018
We wanted to track epithelial cell proliferation in the intestine, so we injected BrdU into mice and used this antibody to track cells that ...
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Great Alternative for PE/Cy7
(1)
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Miltenyi Biotec
February 08, 2018
The goal of this experiment was to analyze the frequency of gdT cells among CD3+CD4-CD8- T cells in the lung.
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Bright And Clean CD45R Antibody
(1)
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Thermo Fisher Scientific
February 08, 2018
This antibody works well to stain CD45R on B cells with little to no optimization and at a decent titration. Other fluorophores may work at ...
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Mounting Medium For Immunofluorescence
(1)
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February 07, 2018
The general aim of the experiment is to study the effect of high fat diet on Pyk2 & Mac3 co-localization. Pyk2 is a nonreceptor tyrosine ...
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Excellent Glut-1 Staining On Human PBMCs
(1)
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Abcam
February 07, 2018
The aim of this study was to observe upregulation of various activation markers upon CD3/CD28 stimulation of purified human CD4+ T cells. I ...
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Compensation Control In Flow Cytometric Analysis
(1)
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Thermo Fisher Scientific
February 07, 2018
The OneComp eBeads™ Compensation Beads were used as a single-color compensation controls for the BD LSR II instrument when running flow ...
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Anti-Mouse Podoplanin Antibody
(1)
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BioLegend
February 07, 2018
In this experiment we were examining type II alveolar epithelial cells using podoplanin in WT and KO mice.
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Well Designed and Thought Through Spectrophotometer
(1)
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DeNovix Inc.
February 06, 2018
We used this instrument a lot in the process of production of different proteins to make vaccines. Quick measurement of DNA and protein ...
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The Antibody Stains EPCAM+ Epithelial Cells Very Well
(1)
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BioLegend
February 06, 2018
We wanted to examine the intestinal epithelial cells in the diseased vs healthy state, so we used this antibody to stain the intestinal ...
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Use Of CD11b Monoclonal Antibody (M1/70), PE To Purity Check Primary Microglial Cultures
(1)
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Thermo Fisher Scientific
February 06, 2018
Single cell suspensions were isolated from brain tissue of day 1-3 C57Bl/6 pups, incubated for 7 days with human M-CSF to enrich for primary...
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Excising DNA Bands From 1.2% Agarose Gels
(1)
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February 06, 2018
Damage free excision of DNA bands required for sensitive cloning applications (wherein no mutations in downstream constructs are required) ...
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Use Of TNF alpha Monoclonal Antibody (MP6-XT22), EFluor 450 To Phenotype Polarized CD4+ T Cells
(1)
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Thermo Fisher Scientific
February 05, 2018
T cells were isolated from C57Bl/6 wild type mice and polarized to a Th17 phenotype using cytokines and antibodies and verified via flow ...
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Pan Ubiquitin Antibody (For Western Blot)
(1)
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Santa Cruz Biotechnology, Inc.
February 05, 2018
This antibody was used to examine the effects of various nutrient treatments on protein ubiquitination in H4IIE whole cell lysates. This ...
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TCR pan gamma delta PE Cy7 Antibody
(1)
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Beckman Coulter Life Sciences
February 05, 2018
To test if Beckman's anti-human TCR gd PECy7 works, I stained human PBMC with this antibody and did a serial titration. This trial ...
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The Kit Very Clearly Stains Dead/Dying Cells Very Well
(1)
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Roche
February 05, 2018
We wanted to examine cell death in a diseased vs healthy state, so we used this kit to label the nuclei of cells that were dead/in the ...
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Anti-mouse CD11b Antibody
(1)
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Thermo Fisher Scientific
February 02, 2018
In this experiment we were examining stem cells in the bone marrow of WT and KO mice.
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Exceptionally Bright IL-10 Antibody
(1)
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BioLegend
February 02, 2018
Our goal was to establish conditions for highly reliable cytokine staining in a high throughput setting. I tested the same clone of anti-IL-...
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Amplification Of DNA Inserts For Cloning And Construction Of Plasmid Vectors
(1)
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Bio-Rad
February 02, 2018
I routinely preform large scale cloning experiments and construction of several plasmid vectors. For this purpose, DNA inserts needs to be ...
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Antibody Stains CD31+ Cells Very Well
(1)
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Cell Signaling Technology
February 01, 2018
We wanted to look at blood vessel formation/organization in a healthy vs diseased state, so we used this antibody to stain for CD31 to ...
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TCR Va7.2 BV605 Antibody
(1)
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BioLegend
February 01, 2018
To test if Biolegend's anti-human va7.2 TCR BV605 works, I stained human PBMC with this antibody and did a serial titration. This trial ...
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Bioruptor For Protein Extraction
(1)
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February 01, 2018
I'm interesting in understanding the molecular mechanism of hedgehog signaling pathway. In this experiment, my goal is to identify binding ...
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DNA Sequencing Service
(1)
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January 31, 2018
Recently we switch DNA sequencing from our core to Genewiz. The service is great with high quality. Just need to request online and drop off...
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PE Anti-Human CD269 (BCMA) Antibody
(1)
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BioLegend
January 31, 2018
Purchased antibody to identify BCMA-positive cells differentiated from normal B cells by FACS. We tested the antibody in several multiple ...
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Anti-Mouse IL-5Ra (CD125) Antibody
(1)
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BD Biosciences
January 31, 2018
In this experiment we were examining stem cells in the bone marrow of WT and KO mice.
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CD161 BV785 Antibody
(1)
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BioLegend
January 30, 2018
To test if Biolegend's anti-human CD161 BV785 works, I stained human PBMC with this antibody and did a serial titration. This trial ...
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Excellent Cell Imaging Multi-Mode Reader
(1)
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Agilent Technologies
January 30, 2018
This is an excellent all in one piece of table top fit equipment every lab or department should own. It does everything from simple ...
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Using Brefeldin A Solution (1000X) To Study Intracellular Cytokine Production
(1)
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Thermo Fisher Scientific
January 30, 2018
Cells were incubated for 5 hours in a stimulation medium consisting on PMA and Ionomycin in order to stimulate cytokine production. ...
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Anti-CD34 Antibody In Bone Marrow
(1)
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Thermo Fisher Scientific
January 29, 2018
In this experiment, we were comparing stem cell populations in bone marrow of WT and KO mice.
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A Robust Hot Start DNA Polymerase For PCR
(1)
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Bioline
January 29, 2018
Non specific background amplification is a major problem during the setting up of a PCR reaction at normal room temperature. IMMOLASE DNA ...
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Alexa Fluor® 647 Anti-Mouse IL-17A Antibody To Phenotype CD4+ Th17 Cells After Polarization
(1)
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BioLegend
January 29, 2018
CD4+ T cells were extracted from the spleens and lymph nodes of mice, selected for CD4 using a negative selection column, and then incubated...
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Western Blot
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Cell Culture
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Concentration
HPLC
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Microarray
Mutagenesis
FISH
CRISPR
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In Vivo Imaging
EMSA
Cell Fractionation
Immunoassay
In Vitro Transcription
ChIP-Seq
Northern Blot
3D Imaging
RNAi
ELISPOT
PCR Cloning
Particle Analysis
Southern Blot
Protein Modification
DNA Analysis
Expression
Cell-Based Assay
Gene Editing
In Vivo Testing
Protein Labeling
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Human
Mouse
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Non-Human Primate
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Arabidopsis
Yeast/Fungi
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Virus
Other Invertebrate
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Drosophila
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Tissue
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Adipose
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Umbilical Cord
Umbilical Vein
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Cell Type
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Adipocyte
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CD8+ T Cell
Competent Cells
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Induced Pluripotent Stem Cell
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Macrophage
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Microglia
Microvascular Endothelial Cell
Monocyte
Myoblasts
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Neuron
Neutrophil
NK Cell
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Oligodendrocytes
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PBMC
Platelets
Progenitor Cell
Red Blood Cell
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Smooth Muscle Cell
Splenocyte
Stem Cell
Stromal Cell
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T Cell
Thymocytes
Tumor Cells
Cell Line
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HeLa
PC3 (PC-3)
HEK293
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CHO
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U2OS
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HL60 (HL-60)
A375 (A-375)
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MCF-7
A431
MDA-MB-231
A549
N2a
Daudi
HCT-116
Raji
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