Santa Cruz Biotechnology
ERK 1 Antibody (G-8)
sc-271269
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ERK operates at the downstream point of MEK and RAFs. As the consequence of the ligand binding or hyperactivation due to mutations, ERK can relay the messages in the nucleus and activate MAPK pathway induced genes. It plays a crucial role in cell proliferation, differentiation, and apoptosis. We used ERK as the loading control for phosphoERK levels in Western Blots in Hela cells.
Western Blot
Hela whole cell Lysates
1:500 dilution prepared in blocking solution, incubated overnight at 4 degrees
5% skimmed non-fat milk in PBST20
1:5000 anti-mouse HRP antibody, incubated for an hour at RT
None
ECL based detection was used
We observed uniform expression levels of ERK in all samples.
N/A
Specificity and reactivity
Nothing
It worked really well for Western Blotting.