Excellent Antibody

Department of Microbiology and Immunology
University of Illinois, Chicago
PostDoctoral Research Fellow

Overall

Quality of Results

Ease-of-Optimization

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Company:

MilliporeSigma

Product Name:

Anti-Hsp72 Antibody, clone 3G7

Catalog Number:

MABE973

Browse Similar Products: Hsp72 Western Blot Antibodies

Heat shock-related 70 kDa protein 2/ HSPA2 is heat shock protein that acts as a molecular chaperone to stabilize preexisting proteins during formation and protect them from aggregation in the cytoplasm. We detected its endogenous levels in different cell lines.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Various nuclear lysates of different cell lines

Primary Incubation

1:1000 dilution prepared in blocking solution, incubated overnight at 4 degrees

Blocking Agent

5% skimmed non-fat milk in PBST20

Secondary Incubation

1:10000 anti-rabbit HRP antibody, incubated for an hour at RT

Tertiary Incubation

None

Detection

ECL based detection was used

Results Summary

We found a specific band at 70 KDa without any noise background.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

Specificity and reactivity

The Bad

Nothing

The Bottom Line

In conclusion, it is a great antibody.

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