Santa Cruz Biotechnology
PKAα/β/γ cat Antibody (B-4)
sc-365615
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PKA gets activated when AMP binds to the two regulatory subunits of the tetrameric PKA holoenzyme which results in the release of active catalytic subunits. We determined PKA levels in HCT116 cells by Western blotting.
Western Blot
Cell Lysates of HCT116
1:500 dilution prepared in blocking solution, incubated overnight at 4 degrees
5% BSA in PBST20
1:5000 anti-mouse HRP antibody, incubated for an hour at RT
None
ECL based detection was used
We observed PKA expression in all samples. This antibody showed specificity and high affinity in Western blotting.
N/A
Specificity and reactivity
Nothing
It is a good antibody for Western Blotting.