Time Course Effect Of Chemoattractant On p44/42 MAPK

March 19, 2018

Internal Medicine
Texas Tech University Health Science Center
Postdoctoral Researcher

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Company:

Cell Signaling Technology

Product Name:

Phospho-p44/42 MAPK

Catalog Number:

4370

MAPK is a serine/threonine kinase involved in cellular program such as cell proliferation, differentiation,motility & death. p44/42 signaling pathway actvated by various stimuli like growth factors, mitogen and cytokines. Here we checked the time course effect of chemoattractant on P-p44/42 phosphorylation.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Mouse lysate

Primary Incubation

1:1000 dilution prepared in 1x TBST

Blocking Agent

5% BSA prepared in 1x TBST

Secondary Incubation

1:4000 dilution prepared in 1x TBST

Tertiary Incubation

None

Detection

ECL

Results Summary

We found there is no significant change in response to chemoattractant.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

This antibody is very good for western blot experiment

The Bad

None

The Bottom Line

This antibody is very specific and works great for western blot. Highly recommended.

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