Abcam
Anti-Hsp70 antibody [5A5]
ab2787
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HSP70 is a member of a family of the proteins called HSP70 family. In co-operation with other proteins, it stabilizes proteins and prevents aggregation in the cytosol and other organelles. We sought to determine its expression levels in different cell lines lysates: Hela, MCF7 and SKBR3.
Western Blot
Various whole cell lysates of different cell lines
1:1000 dilution prepared in blocking solution, incubated overnight at 4 degrees
5% skimmed non-fat milk in PBST20
1:5000 anti-mouse HRP antibody, incubated for an hour at RT
None
ECL based detection was used
We detected HSP70 protein at 70 KDa in Western blotting.
N/A
Specificity and reactivity
Nothing
Overall, its a good antibody for Western Blotting usage.