Reliable CD13 antibody

March 12, 2018

Cell Biology
Albert Einstein College of Medicine
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Biolegend

Product Name:

APC/Cy7 anti-human CD13 Antibody

Catalog Number:

301709

The general aim of the research is to study hematopoiesis and acute myeloid leukemia. This antibody was to measure the surface marker expression on the leukemic cell line THP-1.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

THP-1 cell line

Primary Incubation

1:50 dilution for 30 minutes on ice

Blocking Agent

Human Fc block

Secondary Incubation

None

Tertiary Incubation

None

Detection

Flow cytometry on a BD Aria unit

Results Summary

This antibody detected a clear population of THP-1 cells expressing CD13.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Reliable antibody

The Bad

None

The Bottom Line

Recommended for detection of CD13 on human blood cells.

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