CD132 Flow Cytometry Antibody for Human PBMCs

Microbiology Immunology
University of Texas Medical Branch
Research Scientist

Overall

Quality of Results

Ease-of-Optimization

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Company:

Biolegend

Product Name:

PE anti-human CD132 (common γ chain) Antibody

Catalog Number:

338606

Antibody works great and it is optimized to work on human PBMCs. The goal was to look at CD132 expression on myeloid cells.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Human PBMCs

Primary Incubation

Used at 1:100 dilution for 30 min on ice

Blocking Agent

PBS with 5% BSA

Secondary Incubation

None

Tertiary Incubation

None

Detection

Flow cytometry

Results Summary

Antibody successfully stained myeloid cells, controls were used for non specific binding.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

Works great

The Bad

None

The Bottom Line

We are repeat buyers of this antibody. It works and gives excellent results.

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