Biolegend
Brilliant Violet 650™ anti-mouse CD19 Antibody
115541
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Brilliant Violet 650™ anti-mouse CD19 Antibody has very low non-specific staining, fixes well with both 2% Formaldehyde, 4% Formaldehyde and fir Fix/Perm Foxp3 kit from ebioscience. There is no disintergration of antibody or loss of fluorescence intensity. It stains very brightly at high dilutions as well.
Flow Cytometry
Influeza infected mouse splenocytes
Dilution: 1:400 in Facs Buffer. 20-30 minutes incubation on ice.
anti CD16/32 Fc Block and NMS
No secondary incubation of antibody required
No tertiary incubation of antibody required
405nm Violet Laser, 630 LP Mirror, 660/20 BP Filter
Brilliant Violet 650™ anti-mouse CD19 Antibody from Biolegend stained brightly with minimal background. It works at high dilutions. It doesnt lose fluorescence intensity or disintegrate in fixing with 2% or 4% Formaldehyde or with the Foxp3 Fix/Perm kit from eBioscience.
N/A
Bright, doesn't lose fluorescence, minimal compensation problems with other channels
Brilliant Violet Line of Abs are expensive
This is my go-to antibody for CD19 staining for mouse cells.