Biolegend
Brilliant Violet 711™ anti-human CD3 Antibody
300463
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Browse Similar Products: CD3 Flow Cytometry Antibodies
The aim of this study was to observe CD3 downregulation on CD4+ T cells infected with HIV encoding various Nef alleles. I chose this product as Biolegend offers good quality antibodies at good price.
Flow Cytometry
Human PBMC (CD4+ isolated)
1:100 (50 ul), 30min, 4deg
NA
BD Fortessa-X20
We can observe downregulation of CD3 expression on CD4+ T cells infected with HIV encoding SIV Nef proteins but not their HIV-1 homologs.
Good staining at high dilution
None
I would recommend this antibody for flow cytometry.