Cell Signaling Technology
p53 Antibody
#9282
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p53 is a tumor suppressor protein that is involved in DNA damage, cell cycle and apoptotic pathway. It prevents uncontrolled growth and cancer progression. We detected its levels in 293 T cells upon treatment with drugs of our interest.
Western Blot
Whole cell extracts of 293T
1:1000 dilution prepared in blocking solution, incubated overnight at 4 degrees
5% BSA in PBST20
1:5000 anti-rabbit HRP antibody, incubated for an hour at RT
None
ECL based detection was used
We observed a thick band of p53 in the cell lysates that decreased upon drug treatment.
N/A
Specificity and reactivity
Overall, its a great antibody for p53 detection by Western Blotting.