Cell Signaling Technology
eNOS Antibody
#9572
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Endothelial nitric-oxide synthase (eNOS) is an important enzyme thatcatalyzes the production of nitric oxide (NO), a key regulatorof angiogenesis. Several protein kinases including Akt and PKA can activate this protein and contributes towards angiogenesis. We determined eNOS levels in the HUVEC cells after treatments with the drug of our interest.
Western Blot
Whole cell lysates
1:1000 dilution prepared in blocking solution, incubated overnight at 4 degrees
5% BSA in PBST20
1:2000 anti-rabbit HRP antibody, incubated for an hour at RT
None
ECL based detection was used
Overall, its a great antibody that gave very clean bands of appropriate size.
N/A
Specificity and reactivity
Nothing
In conclusion, its a great antibody for eNOS detection.