Good CD69 Staining On Human PBMCs

March 14, 2018

Infection and Immunity
University College London
PhD Student

Overall

Quality of Results

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Company:

Biolegend

Product Name:

APC/Fire™ 750 anti-human CD69 Antibody

Catalog Number:

310945

The aim of this study was to observe CD69 upregulation on CD4+ T cells infected with HIV encoding various Nef alleles. I chose this product as Biolegend offers good quality antibodies at good price.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Human PBMC (CD4+ isolated)

Primary Incubation

1:100 (50 ul), 30min, 4deg

Blocking Agent

NA

Secondary Incubation

NA

Tertiary Incubation

NA

Detection

BD Fortessa-X20

Results Summary

We can observe different levels of CD69 upregulation on infected CD4+ T cells depending on HIV Nef strain.

DOI or PMID #

NA

Additional Notes

NA

Related Categories

Image Gallery

Summary

The Good

Good staining at high dilution

The Bad

None

The Bottom Line

I would recommend this antibody for flow cytometry.

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