BD Biosciences
Alexa Fluor® 647 Rat Anti-Mouse S100A9 (MRP-14) Clone 2B10 (RUO)
565833
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The goal of the experiment was to detect S100a9 expression in mouse splenocytes. This product was selected because AF647 is a bright stain giving a good separation between positive and negative populations.
Flow Cytometry
C57 mouse splenocytes
1:100 in permeabilization buffer, 25 mins, room temperature
Mouse Fc block
None
Red laser on CytoFlex LX
Intracellular staining of mouse splenocytes showed that nearly all cells were positive for S100a9 expression.
N/A
Easy to use, can be used at a higher dilution than 1:100
Separation between negative and positive populations is not that great.
Good antibody for detecting S100a9 expression in mouse cells.