Labeling CD64 Positive Cells for Flow Cytometry

Medicine
UCSF
Research Associate

Overall

Quality of Results

Ease-of-Optimization

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Company:

Biolegend

Product Name:

Alexa Fluor® 647 anti-mouse CD64 (FcγRI) Antibody

Catalog Number:

139322

This is a reliable antibody for labeling CD64 positive cells in mice. We use it to differentiate macrophage populations in mouse lungs.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Sngle cell suspension of mouse lung cells

Primary Incubation

30 minutes, 4 degrees rocking, 1:100

Blocking Agent

Fc block 1:50 10 million cells/ml, 10 minutes

Secondary Incubation

None

Tertiary Incubation

None

Detection

LSR

Results Summary

The way CD64 was expressed in our sample made the plot a bit smeary, but this was expected because the protein is expressed in different quantities. It reliably labelled the population we were looking for and worked for our purposes.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

Works well

The Bad

Nothing

The Bottom Line

Works reliably and was helpful for our experiment! Easy to use.

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