Excellent Antibody for Detecting Ki-67 in Mouse Cells

Cancer Immunology and Virology
Dana-Farber Cancer Institute
Research Fellow

Overall

Quality of Results

Ease-of-Optimization

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Company:

Invitrogen Antibodies

Product Name:

Ki-67 Monoclonal Antibody (SolA15), PE-eFluor 610, eBioscience™

Catalog Number:

61-5698-80

The goal of this experiment was to identify cells with expression of Ki-67 in the mouse lung. This product was selected because it is compatible with the Foxp3 Fixation/Permeabilization kit. Other available clones for detecting Ki-67 require methanol pre-treatment.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

C57 mouse lung cells

Primary Incubation

1:500, 30 mins, 4 C.

Blocking Agent

Mouse Fc Block

Secondary Incubation

None

Tertiary Incubation

None

Detection

Yellow-Green laser on BD Fortessa X-20

Results Summary

Great separation of Ki-67+ (proliferating) cells from non-proliferating cells.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

Can be used at low concentration, compatible with Foxp3 permeabilization kit, available in many other fluorochrome conjugates!

The Bad

None

The Bottom Line

Exceptional antibody for detecting Ki-67+ cells! Definitely recommended!

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