Great Antibody to Measure c-MYC levels via Flow Cytometry

March 08, 2018

Oncology
Johns Hopkins School of Medicine
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Cell Signaling Technologies

Product Name:

c-Myc (D84C12) Rabbit mAb (Alexa Fluor® 488 Conjugate)

Catalog Number:

12855

We are an immunology lab that studies T cell metabolism. One key factor that has been shown to control T cell metabolism is the transcription factor c-MYC. We were interested in assessing c-MYC levels during CD8 T cell differentiation in different cell populations. We were able to identify two populations with varying levels of c-MYC.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Murine T cells

Primary Incubation

1:250 at RT for 45 minutes (must be done as an intracellular stain)

Blocking Agent

None

Secondary Incubation

None

Tertiary Incubation

None

Detection

Flow cytometer

Results Summary

We were able to identify two populations expressing different levels of myc on a per cell basis in our in vivo model.

DOI or PMID #

27064374

Additional Notes

N/A

Image Gallery

Summary

The Good

Strong antibody to distinguish varying amounts of MYC

The Bad

Higher dilution needed to see the difference

The Bottom Line

Great antibody to measure the master transcription factor that regulates metabolism via flow cytometry. Highly recommend.

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