Anti-Human CD25 Antibody For Flow Cytometry

March 19, 2018

Pathology
Albert Einstein College of Medicine
PhD Candidate

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

PE anti-human CD25 Antibody

Catalog Number:

302606

Our lab focuses on the activation of immune cell subsets in understanding how to kill infected cells of virus. To do this, a strong panel of antibodies is needed to accurately stain and analyze particular cell populations from human peripheral blood mononuclear cells.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Human peripheral blood mononuclear cells

Primary Incubation

5ul antibody on ice for 30 minutes

Blocking Agent

None

Secondary Incubation

None

Tertiary Incubation

None

Detection

Flow cytometry

Results Summary

This antibody readily stains CD25+ cells and is easy to work with despite it being conjugated to a less-common fluorophore.

DOI or PMID #

N/A

Additional Notes

PE is bright, so don't crank up the voltage in this channel too high

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Summary

The Good

Easy staining, clear distinction the population of interest

The Bad

None

The Bottom Line

Highly recommend if staining CD25+ populations in human PBMCs

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