Time Course Effect Of Chemoattractant On eNOS Expression

Internal Medicine
Texas Tech University Health Science Center
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
Write a Review

Company:

BD Biosciences

Product Name:

Anti-eNOS/NOS Type III

Catalog Number:

610296

Nitric oxide (NOS) is a cell specific enzyme that catalyzes the synthesis of NO. eNOS is primarily responsible for the generation of nitric oxide in vascular endothelium. Here we checked the time course effect of chemoattractant on eNOS expression.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Mouse lysate

Primary Incubation

1:1000 dilution prepared in 1x TBST

Blocking Agent

5% milk with 1x TBST

Secondary Incubation

1:4000 dilution prepared in 1x TBST

Tertiary Incubation

None

Detection

ECL

Results Summary

There is no significant increase in eNOS expression by chemoattractant.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

This antibody is very good for western blot experiment

The Bad

None

The Bottom Line

This anibody works great and it is very good for western blot. Highly recommended.

Share your experience with other scientists. Write a Review! »

Join the discussion