R&D Systems
Human/Mouse/Rat PEDF R/PNPLA2 Antibody
AF5365
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The aim is to detect PEDFR expression in mouse skin cells. The data sheet claims that this antibody detects human, mouse, and rat PEDF R/PNPLA2 in direct ELISAs and Western blots. In this experiment I tested it for indirect immunofluorescence staining in frozen mouse skin sections.
Immunofluorescence
Mouse skin
45min, RT, 10ug/ml,
Donkey serum
Donkey anti-sheep Alexa Fluor 594 for 45 min at room temperature
None
All cells in mouse skin seemed to be stained positive including epithelial cells, hair follicles, and fibroblasts. No staining was observed in negative control, so the positive staining seems to be specific.
N/A
It seems to work for an immunofluorescence application.
To be determined
It seems to work in immunofluorescence application, but may need more testing.