Abcam
Anti-LRP6 antibody
ab134146
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The aim is to detect LRP6 expressing cells in mouse skin. The data sheet claims that this antibody works for mouse tissue and could be used in immunofluorescence staining, so I tested it. The results seemed to confirm the application.
Immunofluorescence
Mouse skin
1/200 dilution, RT, 45 min
Goat serum 10%
Goat anti-rabbit Alexa Fluor 594
None
Fluorescence
The epidermis, hair follicles and probably fibroblasts were all stained positive. Negative control without using the antibody showed no positive staining. Therefore, the staining seems to be specific.
N/A
Easy to use, specific
It seems to stain LRP6 in mouse skin well.