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Date Published
Great for Magnetic Separation
(1)
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October 27, 2022
My project involved activating T cells in vivo and it works best after isolating the T cells with magnetic bead separation using this magnet...
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APC Anti-Human CD8 Flow Cytometry Antibody
(1)
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Miltenyi Biotec
October 27, 2022
I wanted to stain the expression of CD8 on the surface of peripheral T cells, so I therefore stained human PBMCs in serial dilutions from 1:...
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Single Mix to Insert Small Mutations into Plasmids
(1)
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New England Biolabs
October 27, 2022
Small plasmid modifications (point mutations, insertion/deletions..) requires serial steps to obtain the final construct. This kit allows ...
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VioBlue Anti-Human CD4 Flow Cytometry Antibody
(1)
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Miltenyi Biotec
October 27, 2022
I wanted to stain the expression of CD14 on the surface of peripheral T cells and monocytes, so I therefore stained human PBMCs in serial ...
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Freeze Media for Human Cells and Tissue Preservation
(1)
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October 27, 2022
CryoStor CS10 has already 10% DMSO, which to protect the sample during the freezing/thawing process and the storage at -80/-196 degrees. No ...
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Excellent Assembly Master Mix for Cloning
(1)
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New England Biolabs
October 26, 2022
We perform a lot of cloning and plasmid modification, and often we are limited by the absence of proper restriction site in the receiving or...
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Best Medium to Increase Transfection Efficiency
(1)
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October 26, 2022
I am using Opti-MEM when I need to transfect cells since it has a unique formulation of ingredients that allows for a reduction of Fetal ...
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FITC Anti-Human CD3 Flow Cytometry Antibody
(1)
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BioLegend
October 26, 2022
I wanted to stain the expression of CD3 on the surface of peripheral T cells, so I therefore stained human PBMCs in serial dilutions from 1:...
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Great Loading Control Antibody
(1)
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Thermo Fisher Scientific
October 26, 2022
I used this tubulin antibody as a cytosolic marker for cellular fractionation experiments. This was a terrific antibody with no background ...
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BUV737 Anti-Human CD1c Flow Cytometry Antibody
(1)
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BD Biosciences
October 26, 2022
I wanted to stain the expression of CD1c on the surface of cDC2 cells, so I therefore stained human PBMCs in serial dilutions from 1:25, 1:...
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Very Good Antibody Against Mouse Nephrin (NPHS1)
(1)
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R&D Systems, a Bio-Techne Brand
October 26, 2022
We work on kidney and renal podocytes development. The podocytes marker Nephrin (NPHS1) is crucial to identify and follow the maturation and...
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Rapid and Reliable Isolation Kit for Human NK Cells
(1)
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October 26, 2022
In our research we need a pure and untouched NK population from human blood sample. This kit allowed us to drastically reduce the time ...
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Great Loading Control GAPDH Antibody
(1)
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GeneTex
October 25, 2022
This GAPDH antibody from GenTex was a great, easy to use loading control and cytosol marker for western blot. High signal, low background. I...
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Efficient Kit for Homemade Bacteria
(1)
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October 25, 2022
Very good alternative to generate homemade, chemically competent E. Coli stocks. We switch to this product from home brewed protocols base ...
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Great Cryomold for Embedding Mouse Lungs
(1)
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Sakura Finetek U.S.A., Inc., Sakura Finetek Inc
October 25, 2022
We embed mouse tissue, especially lungs. Then we analyzed the uptake of our nanoparticles using immunofluorescence and we quantified with ...
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Poor GAPDH Antibody
(1)
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Santa Cruz Biotechnology, Inc.
October 25, 2022
Used for WB as loading control: tons of excessive banding. Do not recommend this as a control antibody.
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Good Basal Medium Supplement for Primary Cell Culture
(1)
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October 25, 2022
We bought this Gibco™ Insulin-Transferrin-Selenium (ITS-G) as a basal medium supplement for primary epithelia cell culture. The reagent is ...
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Great Magnet for Many Samples
(1)
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October 25, 2022
My project involves activating T cells in vivo and I need to purify my samples using magnetic bead isolation before doing so. This magnet ...
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Great Nuclear Marker
(1)
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Santa Cruz Biotechnology, Inc.
October 25, 2022
Was using this anti u1 snRNP70 ab for a experiment where I needed the nuclear fraction of cells. This was an excellent marker easy to ...
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Easy to Use and Fast T Cell Purification
(1)
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October 24, 2022
My project studies activation of T cells, so I need to get a isolated population of T cells to plate and stimulate. This kit is quick and ...
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Fast and Precise Isolation Kit
(1)
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October 24, 2022
Our study required ultra pure and untouched murin lymphocytes T and we previously used columns based purification. With this kit, we speed ...
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Great Ultrasonic Cleaning Bath for Cleaning Tools
(1)
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Branson Ultrasonics Corp.
October 24, 2022
We use a lot of dissection tools for human tissues and they need to stay very clean and sharp, so we use this cleaner to keep them pristine.
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Excellent Transfection Reagent in Many Cell Types
(1)
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October 24, 2022
We used different transfection protocols, according to the cell line requirements. We then switched to JetPrime since it is very versatile ...
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Affordable Mouse Syringe
(1)
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October 24, 2022
I used these syringes for injecting my solutions into the mice intraperitoneal. I had some problems overcoming the skin layer.
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Great T Cell Activator
(1)
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Miltenyi Biotec
October 24, 2022
My project studies the downstream transcription factor changes after T cell activation through the TCR, so this kit is perfect for ...
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Great Solution to Check Western Blotting
(1)
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MilliporeSigma
October 24, 2022
The Ponceau S solution is a fast and easy way of verifying the successful transfer of your proteins to your Western blot membrane. We use it...
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Good Phospho-STAT3 Antibody
(1)
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Cell Signaling Technology
October 21, 2022
To determine whether IL-6 gene transfer affects phosphorylated STAT3 expression. The results showed IL-6 enhanced STAT3 protein ...
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Accurate pH Electrode
(1)
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October 21, 2022
This pH electrode works very well- As with all the pH meters, you can calibrate using the following pH values: 4, 7 and 10.
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Efficient ACK Lysing Buffer
(1)
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October 21, 2022
ACK (Ammonium-Chloride-Potassium) Lysing Buffer is used for the lysis of red blood cells in samples containing white blood cells, such as ...
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Cell Trace CFSE Cell Proliferation Kit
(1)
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October 21, 2022
CFSE is a versatile tool for the fluorescent intracellular labeling of live cells. Labeled cells can be assayed using flow cytometry and ...
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Extremely Reliable and Clean LTB4-R1 Antibody
(1)
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Merck
October 21, 2022
Used to study GPCR trafficking in mouse neutrophils. Reduction in signal is consistently seen in response to cells exposure of LTB4R1 ...
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High Quality Microcentrifuge
(1)
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October 21, 2022
This microcentrifuge works very well. Remember always to keep the temperature at RT, otherwise condensing can cause problems to the machine.
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Great CD4 Antibody for IF
(1)
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BioLegend
October 20, 2022
We wanted to detect helper CD4 T cell infiltration in kidney allografts. Therefore we stained for this antibody.
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PE Anti-Human NKG2A Flow Cytometry Antibody
(1)
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Miltenyi Biotec
October 20, 2022
I wanted to stain the expression of NKG2A on the surface of CD56bright NK cells in some different contexts, so I therefore stained human ...
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Great Calcein AM Cell Viability Dye
(1)
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October 20, 2022
Calcein AM is a cell-permeant dye that can be used to determine cell viability in most eukaryotic cells. In live cells, the nonfluorescent ...
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Efficient GlutaMAX Cell Culture Supplement
(1)
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October 20, 2022
GlutaMAX supplement is an improved cell culture supplement that can be used as a direct substitute for L-glutamine in your cell culture ...
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Invitrogen PE Anti-Mouse FOXP3 mAb Review
(1)
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Thermo Fisher Scientific
October 20, 2022
To titrate the antibody, 2 x 10^6 splenocytes from C57BL/6 mouse were stained with: PerCP-Cy5.5 anti-CD4 Ab and Zombie NIR in 100 µL PBS at ...
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Pen Strep Cell Culture
(1)
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October 20, 2022
Pen strep is an antibiotic used for cell culture. penicillin and streptomycin are used to prevent bacterial contamination of cell cultures ...
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Great CD8a Antibody for IF
(1)
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BioLegend
October 20, 2022
We stained for CD8a, in order to stain for T cell infiltration in kidney allograft and check their localization.
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Great Sonicator
(1)
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October 19, 2022
I use the sonicator for breaking the cell membranes for my WB- This sonicator can also heat, but you can't impose the temperature.
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Very Good Antibody to Stain Follicular Dendritic Cells
(1)
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BD Biosciences
October 19, 2022
In order to stain Follicular Dendritic Cells in cryosections, we tested this antibody in murine spleen as a positive control.
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Efficient Rapid Gold BCA
(1)
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October 19, 2022
The Pierce™ Rapid Gold BCA Protein Assay Kit is a rapid protein assay that, compared to the standard BCA, has been optimized to perform ...
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Extremely Reliable and Clean C5a-R Antibody
(1)
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Abcam
October 19, 2022
Used extensively studying C5aR1 trafficking in mouse neutrophils. Highly specific, although it does bind to the C5a binding site, so ...
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Good GATA3 Antibody for IF
(1)
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BD Biosciences
October 19, 2022
As a positive control for Gata3 staining, we used the virgin mammary gland, where E-Cadherin+ luminal cells express Gata3.
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Good Signal with PE Anti-Mouse CD254 (TRANCE, RANKL) Antibody
(1)
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BioLegend
October 19, 2022
The study we are conducting revolves around the mechanism of bone resorption in a mouse model of osteosarcoma. With this antibody we are ...
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Af488 Anti-Human CD127 Flow Cytometry Antibody
(1)
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BioLegend
October 18, 2022
I wanted to stain the expression of CD127 on the surface of peripheral T cells and NK cells, so I therefore stained human PBMCs in serial ...
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Great PE Anti-Mouse/Human CD44 Antibody
(1)
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BioLegend
October 18, 2022
This antibody was used to evaluate the expression of PSGL1 in colon carcinoma. Highly sensitive and overall good results obtained.
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Great CD68 Antibody for Flow Cytometry
(1)
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BioLegend
October 18, 2022
This antibody worked very well and allowed the detection of macrophages infiltration in a mouse model of tumor.
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Very Clean Anti-Lactoferrin/LTF Antibody Picoband
(1)
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BosterBio
October 18, 2022
Nicely detects degranulated LTF in supernatant mouse neutrophils stimulated with bacteria. Used at 1:2000 in 5% BSA.
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Neurotensin ELISA on Tissue Lysate
(1)
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RayBiotech
October 18, 2022
Goal was to detect neurotensin level in adipose tissues (both Brown and White adipose tissue). Tissues utilized were brown adipose depot and...
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Light Sources
Liquid Handling Equipment
Luminometer
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Microscopes
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Primary Cells
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Purification Equipment
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Rocker
Sample Prep Equipment
Scanners
Services
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Slides
Software
Sonicator
Spectrometer
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Substrates
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Vacuum Manifolds
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Applications
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Western Blot
Flow Cytometry
ELISA
Immunofluorescence
Immunoprecipitation
Immunocytochemistry
Immunohistochemistry
Block/Neutralize/Inhibit
Cell Isolation
Dot Blot
ChIP
Purification
Staining
Cell Culture
DNA Purification
PCR
RNA Analysis
RNA Purification
qPCR
Clean-up
Microscopy
Cloning
RT-PCR
cDNA Synthesis
Transfection
Electrophoresis
RT-qPCR
Cell Counting
Protein Purification
Next Gen Sequencing (NGS)
Library Construction
Genotyping
Gel Imaging
Protein Assay
Reporter Assay
Cell Lysis
Multiplex
Gel Staining
RNA-Seq
Live Cell Imaging
Cell Differentiation
Ligation
Concentration
HPLC
Sequencing
Chromatography
Mass Spectrometry
Labeling
Microarray
Mutagenesis
FISH
CRISPR
TUNEL Assay
In Vivo Imaging
EMSA
Cell Fractionation
Immunoassay
In Vitro Transcription
ChIP-Seq
Northern Blot
3D Imaging
RNAi
ELISPOT
PCR Cloning
Particle Analysis
Southern Blot
Protein Modification
DNA Analysis
Expression
Cell-Based Assay
Gene Editing
In Vivo Testing
Protein Labeling
Other
Species
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Human
Mouse
Rat
Non-Human Primate
Rabbit
Sheep
Hamster
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Horse
Monkey
C. elegans/Worm
Insect
Amoeba/Protozoa
Bacteria/Archaea
Arabidopsis
Yeast/Fungi
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Fish
Virus
Other Invertebrate
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Drosophila
Goat
Rhesus
Tissue
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Adipose
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Blood Vessel
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Brain
Breast
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Dermis
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Eye
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Hair
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Hippocampus
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In Vivo
Intestine
Kidney
Large Intestine
Liver
Lung
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Mammary Gland
Mouth
Muscle
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Pancreas
Peripheral Blood
Peritoneum
Placenta
Prostate
Rectum
Retina
Skeletal Muscle
Skin
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Spinal Cord
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Teeth
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Thyroid Gland
Tonsil
Trachea
Umbilical Cord
Umbilical Vein
Uterus
Vein
Cell Type
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Adipocyte
Astrocyte
B Cell
CD8+ T Cell
Competent Cells
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Embryonic Stem Cell
Endothelial Cell
Eosinophil
Epithelial Cell
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Glial Cell
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Helper T Cell
Hematopoietic Stem Cell
Hepatocyte
HUVEC
Induced Pluripotent Stem Cell
Keratinocyte
Leukocytes
Lymphocyte
Macrophage
Mesenchymal Stem Cell
Microglia
Microvascular Endothelial Cell
Monocyte
Myoblasts
Myocyte
Neuron
Neutrophil
NK Cell
NKT Cell
Oligodendrocytes
Osteoclast
PBMC
Platelets
Progenitor Cell
Red Blood Cell
Regulatory T Cells
Skeletal Muscle Cell
Smooth Muscle Cell
Splenocyte
Stem Cell
Stromal Cell
Synoviocyte
T Cell
Thymocytes
Tumor Cells
Cell Line
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HeLa
PC3 (PC-3)
HEK293
HEK293T (HEK-293T)
CHO
Jurkat
3T3
Caco-2
HepG2
THP-1
U2OS
PC12 (PC-12)
HL60 (HL-60)
A375 (A-375)
MOLT4 (MOLT-4)
MCF-7
A431
MDA-MB-231
A549
N2a
Daudi
HCT-116
Raji
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