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Date Published
Good Beads for Magnetic Isolation
(1)
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Miltenyi Biotec
December 07, 2022
We are an immunotherapy focused group, with interest in CD8 T-cells and their TCRs. For our experimental purposes, we routinely require ...
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Easy Way to Remove CD19+ Cells from PBMC
(1)
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Miltenyi Biotec
December 07, 2022
In our experiment, we used CD19 microbeads to isolate CD19+ cells from human PBMCs (Peripheral blood mononuclear cells). We did use them to ...
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Recommended Scigen Tissue-Plus™ O.C.T. Compound
(1)
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Fisher Scientific
December 06, 2022
I used this to inflate mouse lungs prior to cryo sectioning and staining for confocal microscopy imaging. It keeps the structure of the ...
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Poloxamer Used for Thermo Responsive Hydrogel
(1)
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MilliporeSigma
December 06, 2022
Pluronic F-127 is an interesting Poloxamer that I have been using for the fabrication of a thermo responsive hydrogel. The sol-gel ...
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Good Buffer for Crosslinking Process
(1)
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December 06, 2022
I use MES buffer solution for the crosslinking of proteins onto a collagen-based membrane. I used to prepare my MES buffer, but sometimes ...
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Useful Reagent for Chemical Synthesis
(1)
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MilliporeSigma
December 06, 2022
I use triethylamine in few step of a chemical synthesis where I need to add amine groups to another product in order to finally obtain a ...
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Good Product for Biomimetic Systems
(1)
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MilliporeSigma
December 06, 2022
I used chitosan medium molecular weight for its innate antimicrobial properties. I usually blend it with another biocompatible polymer to ...
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Excellent CD45RO Antibody
(1)
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BioLegend
December 06, 2022
We are an immunotherapy focused group with an interest in CD8 T-cells and their TCRs. For our experimental purposes, we routinely isolate ...
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Reliable Human Chemokine Array
(1)
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RayBiotech
December 06, 2022
My lab studies the pathobiology of Ehrlichia chaffeensis, an obligately intracellular bacterium. I used this kit to characterize the ...
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Good Product for Electrospinning Applications
(1)
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MilliporeSigma
December 05, 2022
I use Poly(ethylene oxide) (PEO) as a copolymer in formulation used for electrospinning. Thanks to PEO I am able to create nanofibrous ...
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Good Counting Beads for Quantifying Populations
(1)
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December 05, 2022
Our lab aims to utilize CD8 T-cell for therapeutical purposes. Our experiments are heavily based on in vitro and in vivo cell-based ...
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Antibody for p-p38
(1)
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Cell Signaling Technology
December 05, 2022
Currently working with pregnancy-related cells (amnion, chorion) and doing protein analysis of different markers upon different treatments (...
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Excellent Acti-Stain 555 Phalloidin
(1)
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December 05, 2022
I use this for very good actin staining on my cells (A549, MRC5, human primary cells, MSCs). I reconstitute this in 500 microliters of ...
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Good Product for Crosslinking
(1)
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MilliporeSigma
December 05, 2022
I am developing a functionalized membrane for the treatment of wound healing. NHS helps me obtaining the crosslinking of proteins together ...
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Great ACLY Antibody for Western Blotting
(1)
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Proteintech Group Inc
December 05, 2022
Used this antibody to probe for levels of ACLY in MCF10a cells - very clear signal with little background noise.
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Antibody for p-38 Total
(1)
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Cell Signaling Technology
December 02, 2022
Currently working with pregnancy related cells and doing protein analysis of different markers upon different treatments (i.e., bacteria ...
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MTHFD2 Antibody for Western Blot
(1)
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BosterBio
December 02, 2022
This antibody of MTHFD2 is good for WB with mouse tissue and human cell. Signal is strong and the background is ok.
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Great Astrocyte Marker
(1)
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MilliporeSigma
December 02, 2022
I have used this antibody to detect astrocyte among a number of neurons within the mouse brain. It has worked very well in mouse sample.
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Quick & Reliable Way to Remove Monocytes and Macrophages from PBMC
(1)
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Miltenyi Biotec
December 02, 2022
In our Lab, we use anti-human CD14 microbeads to purify monocytes and macrophages from human peripheral blood mononuclear cells (PBMC), as ...
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Good Kit for Measuring Editing Efficiency
(1)
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New England Biolabs
December 02, 2022
We were interested in studying the role of DNA methylation in the differentiation of of CD4 T-cells. CRISPR-Cas9 approaches were used to ...
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Good CD32 Staining on Human Platelets
(1)
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Thermo Fisher Scientific
December 02, 2022
CD32 is expressed on the platelet surface. CD32 Monoclonal Antibody (6C4 (CD32)) PerCP-eFluor 710 allows good staining of CD32 on the ...
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TRIM16/EBBP Antibody
(1)
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Bethyl Laboratories, Inc.
December 01, 2022
The aim of the experiment was to check the levels of TRIM16 in osteosarcoma cell lines. So western blot analysis was done for the same. the ...
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Bright CD62P Staining on Human Platelets
(1)
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BD Biosciences
December 01, 2022
CD62P is present in the granules of resting platelets and expressed on the cell surface following platelet activation. BV480 Mouse Anti-...
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Recommended Fixation/Permeabilization Kit
(1)
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BD Biosciences
December 01, 2022
I use this reagent for fixing cells prior to flow cytometry analysis. Used so far on A549, MRC5, primary human cells, MSCs and lung tissue. ...
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Excellent Transduction Reagent
(1)
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November 30, 2022
Our group is interested in using TCRs for therapeutic purposes. As such, for our experiments we commonly transduced TCR constructs or other ...
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Very Specific Signal with EMP/MAEA Antibody
(1)
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R&D Systems, a Bio-Techne Brand
November 30, 2022
HEK293 cells were lysed with RIPA buffer before spinning down, mixed with loading buffer and run on a 12% Gel, and transferred to PVDF ...
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Excellent One-Step qPCR Kit
(1)
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MilliporeSigma
November 30, 2022
Our group focuses on the utilization of CD8 T-cells as means of cancer treatment. For our experimental purposes and the development of such ...
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Easy to Use Kit to Measure Progesterone
(1)
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Thermo Fisher Scientific
November 30, 2022
Currently working with pregnancy related cells (amnion and chorion), and doing analysis of different analytes upon different treatments.
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Good Quality Antibody for p-JNK
(1)
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Cell Signaling Technology
November 30, 2022
Currently working with pregnancy related cells (amnion, chorion, decidual) and doing protein analysis of different markers upon different ...
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Good TLR9 Staining on Human Platelets
(1)
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Novus Biologicals, a Bio-Techne Brand
November 30, 2022
TLR9 is present in platelet granules and expressed on the cell surface following activation. TLR9 Antibody (26C593.2) [PE] provides good ...
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Good Antibody to Evaluate Neuronal Activity
(1)
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Cell Signaling Technology
November 29, 2022
I have used this antibody to detect activity of neurons from an animal model of inflammatory pain in the brain. It has worked very well in ...
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Great ECL Detection Reagent for Even Harder to Detect Proteins
(1)
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November 29, 2022
The kit is an ultra-sensitive enhanced chemiluminescent substrate for high attogram protein detection. It provides the highest-level ...
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Excellent Antibody for Detecting Total Akt
(1)
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Cell Signaling Technology
November 29, 2022
I am studying the effect of extracellular phospholipid on microglia signalling cascade. In response to the phospholipid, there is an ...
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Good F4/80 Antibody for IF
(1)
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BioLegend
November 29, 2022
I am interested to study myeloid cells in steady state and inflammation. As a positive control for F4/80 I stained the small intestine.
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IgA2 Antibody Works Well
(1)
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Miltenyi Biotec
November 29, 2022
We were interested in establishing IgA1 and IgA2 specific immortalized B-cell lines. PBMCs from blood donors were infected with EBV and once...
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Great Labelling of Acetylcholine Neurons
(1)
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MilliporeSigma
November 29, 2022
I have used this antibody to detect acetylcholine neurons from the mouse striatum. It has worked greatly in mouse sample.
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Good Antibody for Phosphorylated Akt
(1)
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Cell Signaling Technology
November 28, 2022
I am studying the effect of extracellular phospholipid on microglia signalling cascade. In response to the phospholipid, there is an ...
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Easy to Use SDM Kit
(1)
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Agilent Technologies
November 28, 2022
The aim of the experiment was to generate a point mutation in the gene of interest in over expressing plasmid expressing a WT protein using ...
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Great Phospho-EGF Receptor Antibody for Western Blotting
(1)
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Cell Signaling Technology
November 28, 2022
Good antibody that recognises phosphorylation of the EGFR at Y1068, following treatment of MCF10a cells with EGF for 15 minutes.
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Mitochondrial Staining and Immunofluorescence Using MitoTracker Deep Red
(1)
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Cell Signaling Technology
November 28, 2022
Here, we used the MitroTracker Red to stain mitochondria in Human Umbilical Vein Endothelial Cells (HUVEC) to asses mitochondrial dynamics ...
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Great Isotype Control
(1)
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Thermo Fisher Scientific
November 28, 2022
Our research is heavily relied on cell-based assays. We often need to characterize cell lines or primary cells, using FACs, which requires ...
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Very Good APC Anti-Mouse CD32 (Fcgr2) Antibody
(1)
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BioLegend
November 28, 2022
I used this antibody to characterize the NK cells in a CD32b conditional Knock out mice (natural killer cells are missing the CD32b) by ...
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Easy to Use Dye for the Detection of Cell Viability or Proliferation
(1)
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November 23, 2022
The reagent is a high affinity, intracellular calcium indicator that is ratiometric and UV light—excitable that allows users to measure the ...
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Good NF-kB p65 Antibody
(1)
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Cell Signaling Technology
November 23, 2022
Currently working with pregnancy related cells and doing protein analysis of different markers upon different treatments.
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Great Reagent for Detection of Proteins in Western Blot
(1)
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Thermo Fisher Scientific
November 23, 2022
The kit is an ultra-sensitive enhanced chemiluminescent (ECL) substrate for low femtogram protein level detection by western blot analysis ...
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Great Transfection Reagent for Transfecting siRNA
(1)
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November 23, 2022
The reagent provides the highest transfection efficiencies on the widest variety of cell types for siRNA-mediated gene knockdown experiments...
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Excellent Transfection Reagent
(1)
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November 23, 2022
Used this to make ACSS2-knockout MCF10a cells - easy transfection protocol with high efficiency of transfection.
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Very Good PE Anti-Mouse CD16 Antibody
(1)
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BioLegend
November 23, 2022
I used this antibody to characterize the NK cells in a CD16 conditional Knock out mice (natural killer cells are missing the CD16 receptor)....
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Really Good Viability Kit
(1)
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BioLegend
November 22, 2022
I use this antibody to detect my live vs dead lymphocytes when I do FACS analysis. In this particular experiment, I used it on ex-vivo C57BL...
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Very Good Red Blood Cell Lysing Buffer
(1)
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Quality Biological, Inc.
November 22, 2022
I use this buffer to burst red blood cells when I process mouse cell tissues in order to obtain lymphocytes for future analysis.I harvest ...
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Western Blot
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Cell Culture
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Concentration
HPLC
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Microarray
Mutagenesis
FISH
CRISPR
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In Vivo Imaging
EMSA
Cell Fractionation
Immunoassay
In Vitro Transcription
ChIP-Seq
Northern Blot
3D Imaging
RNAi
ELISPOT
PCR Cloning
Particle Analysis
Southern Blot
Protein Modification
DNA Analysis
Expression
Cell-Based Assay
Gene Editing
In Vivo Testing
Protein Labeling
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Human
Mouse
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Non-Human Primate
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Arabidopsis
Yeast/Fungi
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Virus
Other Invertebrate
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Drosophila
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Tissue
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Adipose
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Umbilical Cord
Umbilical Vein
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Cell Type
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Adipocyte
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CD8+ T Cell
Competent Cells
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Induced Pluripotent Stem Cell
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Macrophage
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Microglia
Microvascular Endothelial Cell
Monocyte
Myoblasts
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Neuron
Neutrophil
NK Cell
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Oligodendrocytes
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PBMC
Platelets
Progenitor Cell
Red Blood Cell
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Smooth Muscle Cell
Splenocyte
Stem Cell
Stromal Cell
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T Cell
Thymocytes
Tumor Cells
Cell Line
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HeLa
PC3 (PC-3)
HEK293
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CHO
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U2OS
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HL60 (HL-60)
A375 (A-375)
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MCF-7
A431
MDA-MB-231
A549
N2a
Daudi
HCT-116
Raji
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