Good Antibody for Phosphorylated Akt

November 28, 2022

University of Cambridge

Overall

Quality of Results

Ease-of-Optimization

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Company:

Cell Signaling Technology

Product Name:

Phospho-Akt (Ser473) (D9E)

Catalog Number:

4060

Clone Number:

N/A

I am studying the effect of extracellular phospholipid on microglia signalling cascade. In response to the phospholipid, there is an increase in phosphorylation of Akt at Serine 473 in primary murine microglia. Changes in the expression of phospho Akt were detected using immunoblotting.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Primary murine microglia

Primary Incubation

1:1000, 4 degree C, overnight

Blocking Agent

5% milk

Secondary Incubation

1:5000 anti rabbit

Tertiary Incubation

N/A

Detection

Horseradish peroxidase (HRP) substrate

Results Summary

The antibody was very sensitive and gave a single specific bands. The bands indicated increased expression of phospho AKT in reponse to the extracellular phospholipid in primary murine microglia. This antibody is used to investigate AKT pathway.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Reliable and specific antibody.

The Bad

N/A

The Bottom Line

Good antibody to detect phophorylated Akt.

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