Agilent
QuikChange II Site-directed mutagenesis kit
200523
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The aim of the experiment was to generate a point mutation in the gene of interest in over expressing plasmid expressing a WT protein using the SDM kit. The point mutation plasmid was successfully generated and used further for over expressing in the osteosarcoma cell lines.
Site directed mutagenesis
N/A
Follow the protocol for all the concentrations as mentioned in the kit.
The point mutation (G>C) in the gene of interest was incorporated in the plasmid initially expressing the WT protein successfully and was further used for expressing the mutant protein.
Easy to use, high success rate and detailed stepwise protocol of the kit makes it user friendly.
Highly recommended easy to use Site directed mutagenesis kit.