Antibody for p-38 Total

December 02, 2022

University of Texas Medical Branch Galveston

Overall

Quality of Results

Ease-of-Optimization

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Company:

Cell Signaling Technology

Product Name:

p38 MAPK Antibody

Catalog Number:

9212

Clone Number:

N/A

Currently working with pregnancy related cells and doing protein analysis of different markers upon different treatments (i.e., bacteria infection).

Experimental Design and Results Summary

Applications

Western Blot

Sample

Decidual cells; amnion mesenchymal cells

Primary Incubation

1:1000 dilution, overnight, 4 degree

Blocking Agent

5% nonfat milk in 1× Tris-buffered saline-Tween 20 (TBS-T)

Secondary Incubation

1:10000, 2 hours, RT

Tertiary Incubation

None

Detection

ECL WB; Bio-Rad Image Lab 6.0 software.

Results Summary

The results looks good using this dilution. The band is clear and the run does not have a lot of background.

DOI or PMID #

N/A

Additional Notes

Rabbit; MW: 40kDa

Image Gallery

Summary

The Good

Good quality

The Bad

None

The Bottom Line

Good signal, appropriate results for this marker.

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