Cell Signaling Technology
Phospho-p38 MAPK (Thr180/Tyr182) Antibody
9211
N/A
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Currently working with pregnancy-related cells (amnion, chorion) and doing protein analysis of different markers upon different treatments (i.e. bacteria infection).
Western Blot
Fetal membrane cells
1:1000 dilution, overnight, 4 degree
5% nonfat milk in 1× Tris-buffered saline-Tween 20 (TBS-T)
1:10000, 2 hours, RT
None
ECL WB; Bio-Rad Image Lab 6.0 software.
This dilution worked fine for my samples. The band was good for most of the samples. No background seen in most of them as well.
Rabbit; MW: 43kDa
Good quality; works fine with the cell types tested.
Good antibody; great signal.