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Date Published
Great PI Staining Buffer
(1)
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BD Biosciences
November 10, 2022
The goal of our research is to check the drug effect on the MDA-MB-231 cell cycle. We used this PI staining buffer to stain fixed cell and ...
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Great Anti-STAT3 Antibody for Western Blotting
(1)
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Abcam
November 10, 2022
Used this antibody to detect levels of pSTAT in MCF10a cells, as a readout of EGFR activation. Clear bands with low background.
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Great Phospho-Akt Antibody for Western Blotting
(1)
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Cell Signaling Technology
November 10, 2022
Used this to detect levels of phosphorylated Akt (at T308) in MCF10a cells as a readout for PI3K signalling - it gave a great signal.
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Helpful Membrane Protein Extraction Kit
(1)
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November 10, 2022
A convenient kit for the isolation of native integral membrane and membrane-associated proteins, without the need for ultracentrifugation. ...
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Great GAPDH Antibody for Western Blotting
(1)
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Abcam
November 10, 2022
Used this GAPDH antibody as a loading control for all my western blots - GAPDH is readily expressed in lots of cells and this antibody gives...
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Reliable and Effective HLA-A2 Antibody
(1)
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BioLegend
November 09, 2022
Our research focuses on utilizing CD8+ T-cells and their TCRs therapeutically, the effectivity of which is depended on HLA type.
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Good UCP1 Antibody to Study Thermogensis
(1)
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Cell Signaling Technology
November 09, 2022
We are studying fat metabolism and brown fat thermogenesis. We have tested this antibody with brown fat tissue from mice and a brown fat ...
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Vav Antibody Had Some Background Issues, but Got There in the End
(1)
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ProSci, LLC
November 09, 2022
The antibody was OK, sometimes a bit hit and miss. We didn't have the best positive control, but sometimes lack of signal where you'd expect...
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siRNA Transfection for A Lot of Cell Lines
(1)
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Revvity
November 09, 2022
The Darmafect 1 transfection reagent is our standard reagent for transfecting cells with siRNA e.g. to mediate specific knock-down of genes/...
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Extremely Reliable and Clean KRAS Antibody
(1)
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Proteintech Group Inc
November 09, 2022
Used in cell fractionation experiments as a marker of plasma membrane, very clean signal across multiple experiments.
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Staphylococcus aureus Grows Well, As They Should!
(1)
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MilliporeSigma
November 09, 2022
These bacteria were used in in vitro killing assays, where bone marrow derived neutrophils were incubated with log growth, serum opsonized S...
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Rab5 Antibody Used as a Marker of Early Endosomes for Cell Fractionation
(1)
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Abcam
November 08, 2022
After cell fractionation via differential centrifugation and anti-EEA1 immunoisolation, purity of endosomal fractions were checked with Rab5...
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Great Ser473 Antibody for Western Blotting
(1)
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Cell Signaling Technology
November 08, 2022
Used this antibody to detect levels of phosphorylated Akt as a readout for PI3K signalling in MCF10a cells following EGF stimulation. Used ...
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Histone H3 Antibody Works Well in our Hands
(1)
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Abcam
November 08, 2022
Used to detect Citrullinated histones in purified neutrophils when stimulated with DH5a E. coli. See increasing Citrullination with longer ...
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Extremely Reliable and Clean CD62L Antibody
(1)
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BioLegend
November 08, 2022
Used to study the plasma membrane and total levels of CD62L / L-Selectin in bone marrow derived mouse neutrophils.
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Very Reliable CD16 Antibody
(1)
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BioLegend
November 08, 2022
Used to study the plasma membrane and total levels of CD16 in bone marrow derived mouse neutrophils.
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Excellent and Very Specific Ly-6G Antibody
(1)
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BioLegend
November 07, 2022
Has been used extensively in our lab for the study of mouse neutrophils in isolated bone marrow. Used in countless experiments and never ...
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Great cAMP Measurement Kit for GPCR Screen
(1)
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Promega
November 07, 2022
We are interested in the potential function of an endogenous metabolite in human serum. We use this cAMP-Glo assay kit combined with our ...
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Very Effective PerCP/Cy5.5 Anti-CD45.2
(1)
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BioLegend
November 07, 2022
After doing an adoptive transfer of 1:1 ratio of CD45.1+ and CD45.2+ Natural Killer cells into a double positive B6 mouse, we used the PerCP...
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Very Good Mouse BV510 Anti-CD45.1 Antibody
(1)
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BioLegend
November 07, 2022
After doing an adoptive transfer of 1:1 ratio of CD45.1+ and CD45.2+ Natural Killer cells into a double positive B6 mouse, we used the BV510...
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CD182 Antibody with Very Strong Signal
(1)
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BioLegend
November 07, 2022
Used to study the plasma membrane localization and receptor trafficking of CXCR2. PE signal is very strong.
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Great Akt Antibody for Western Blotting
(1)
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Cell Signaling Technology
November 07, 2022
Used as a readout for PI3K signalling in MCF10a cells following 16 hours of starvation and then 15 minutes of EGF stimulation.
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Brilliant Antibody, Very Clean WB & Excellent IP of Early Endosomes
(1)
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Cell Signaling Technology
November 04, 2022
Used for cell fractionation experiments in mouse neutrophils. Was extremely clean and reliable at IP / organelle isolation of early ...
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Strong Stain, Easy Optimization
(1)
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November 04, 2022
Used to study the release of NETs from neutrophils in response to S. aureus stimulation in vitro. Great signal, enabling the identification ...
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Great ErbB3 Antibody for Immunoprecipitation
(1)
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Cell Signaling Technology
November 04, 2022
Used this antibody to pull down the ErbB3 receptor from MCF10a cells following EGF stimulation. I used the antibody alongside the protein A ...
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Caspase-Glo® 3/7 Assay for Apoptosis Detection
(1)
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Promega
November 04, 2022
We regularly detect apoptosis in several human cancer cell lines using the Caspase-Glo® 3/7 Assay from Promega. The protocol is easy to ...
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Clodronate Liposomes Used to Deplete Alveolar Macrophages in Mice
(1)
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November 04, 2022
Efficient depletion of alveolar macrophages without affecting total neutrophil numbers. In our hands, 72 hours post-treatment saw the most ...
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High Signal Peptide for Study of Agonist Induced Internalization of fMLP Receptor
(1)
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Cambridge Research Systems
November 03, 2022
Excellent tool to visualise the trafficking of the fMLP receptor upon agonist stimulation. Strong signal.
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Very Effective BV605 Anti-NK1.1 Mouse Antibody
(1)
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BioLegend
November 03, 2022
One week after depleting Natural Killer cells from a B6 mouse, we used the BV605 anti-NK1.1 antibody to analyze the lymphocytes in the ...
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Very Effective BV785 KLRG1 Antibody
(1)
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BioLegend
November 03, 2022
We infected B6 mice with Mouse Cetamegalovirus and used the anti-KLRG1 antibody to analyze the Natural Killer cells in the infected mice's ...
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Great Luciferin-Based Quantification of CYP3A4 Activity
(1)
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Promega
November 03, 2022
Cytochrome P450 3A4 (CYP3A4) is an important enzyme in the body, mainly found in the liver and in the intestine. We previously found a small...
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Very Good Mouse FITC Anti-NKp46 Antibody
(1)
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BioLegend
November 03, 2022
One week after depleting Natural Killer cells from a B6 mouse, we used the FITC anti-NKp46 antibody to analyze the lymphocytes in the spleen...
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Great LDH Quantification Kit
(1)
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Cayman Chemical
November 03, 2022
We are interested in the potential function of a secreted small peptide. Preliminary data suggest this peptide may be involved in cell death...
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Great IgG1 Antibody with Good Staining
(1)
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BioLegend
November 03, 2022
We are interested in studying cancer and cancer immunology in the lab. We take the spleen of mice and stain with a panel of antibodies for ...
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Good Cyclin D3 Antibody
(1)
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Cell Signaling Technology
November 02, 2022
This antibody was used to check whether ACSL gene knockdown affects cyclin D3 expression in PC-3 cells. The results showed ACSL could affect...
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Excellent Cell Culture Microplates
(1)
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Agilent Technologies
November 02, 2022
I use these microplates for Seahorse studies on SHSY5Y, A549 and MRC5 cells. Following vendor guidelines for cell seeding and inhibitor ...
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Product Retains High Activity Despite Multiple Freeze Thaw Cycles
(1)
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Roche
November 02, 2022
Used extensively in the study of NETosis from mouse neutrophils, when used at 1mg.mL, effeciently cleaves and thus inhibits NETs.
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ACSS2 Antibody for Western Blot
(1)
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Cell Signaling Technology
November 02, 2022
Used this antibody to confirm knockout in MCF10a cells by western blotting. Used at dilution 1:1000 in 5% BSA in TBST, very clear signal.
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Extremely Reliable and Clean Results with TNF-alpha ELISA
(1)
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R&D Systems, a Bio-Techne Brand
November 02, 2022
Used to study the Zymosan induced TNFa response from isolated mouse neutrophils. Saw robust and reproducible response across many ...
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Great B220 Antibody with Strong Staining
(1)
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BD Biosciences
November 02, 2022
We are interested in studying cancer and cancer immunology in the lab. We take the spleen of mice and stain with a panel of antibodies for ...
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Excellent and Very Specific CD11b Antibody
(1)
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BD Biosciences
November 02, 2022
Very useful for all our flow cytometry experiments where we study Neutrophils in bone marrow cells. Very specific and strong signal, and ...
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Reliable Centrifuge Tubes
(1)
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VWR Scientific Products
November 01, 2022
I use this tube for every step of cell collection, centrifugation, medium preparation. They are sterile and suitable for cell work. Very ...
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Good CD4 Antibody
(1)
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BioLegend
November 01, 2022
We are interested in studying cancer and cancer immunology in the lab. We take the spleen of mice and stain with a panel of antibodies for ...
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Good Cyclin D1 Antibody for Western Blot
(1)
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Cell Signaling Technology
November 01, 2022
This antibody was used to check whether ACSL gene knockdown affects cyclin D1 expression in PC-3 cells. The results showed ACSL could affect...
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Awesome Microscope
(1)
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Thermo Fisher Scientific
November 01, 2022
Our group is interested in CD8 T-cells and their applications in immunotherapy. Most of our assays are in-vitro cell-based, so microscopy is...
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Great CD69 Antibody with Good Staining
(1)
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Thermo Fisher Scientific
November 01, 2022
We are interested in studying cancer and cancer immunology in the lab. We take the spleen of mice and stain with a panel of antibodies for ...
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APC/Cyanine7 Anti-Mouse CD3 Antibody
(1)
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BioLegend
November 01, 2022
In our lab we study mouse Natural Killer cells. We use this antibody to create a 'dump' gate which includes CD3, CD19 and TCRb to gate out T...
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ELISA Kit is Easy to Use and Optimize
(1)
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R&D Systems, a Bio-Techne Brand
October 31, 2022
Used to study the release of cytokines from isolated mouse neutrophils in response to opsonised Zymosan.
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BUV395 Anti-Human CD123 Flow Cytometry Antibody
(1)
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BD Biosciences
October 31, 2022
I wanted to stain the expression of CD123 on the surface of peripheral pDCs, so I therefore stained human PBMCs in serial dilutions from 1:...
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Excellent Anti-Mouse CD3 Antibody (FITCH)
(1)
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Abcam
October 31, 2022
Our work requires is focused on the mouse lymphocytes T population. This antibody provides an excellent marker for FACS staining of T cells.
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Balance / Scale
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Camera
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Cell Sorting Equipment
Cells and Strains
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Chemicals and Reagents
Chromatography Equipment
Columns
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Counter
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Electrophoresis Equipment
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ELISA Kits
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Freezer
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Kits
Labels / Dyes / Stains
Ladders and Markers
Light Sources
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Western Blot
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ELISA
Immunofluorescence
Immunoprecipitation
Immunocytochemistry
Immunohistochemistry
Block/Neutralize/Inhibit
Cell Isolation
Dot Blot
ChIP
Purification
Staining
Cell Culture
DNA Purification
PCR
RNA Analysis
RNA Purification
qPCR
Clean-up
Microscopy
Cloning
RT-PCR
cDNA Synthesis
Transfection
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RT-qPCR
Cell Counting
Protein Purification
Next Gen Sequencing (NGS)
Library Construction
Genotyping
Gel Imaging
Protein Assay
Reporter Assay
Cell Lysis
Multiplex
Gel Staining
RNA-Seq
Live Cell Imaging
Cell Differentiation
Ligation
Concentration
HPLC
Sequencing
Chromatography
Mass Spectrometry
Labeling
Microarray
Mutagenesis
FISH
CRISPR
TUNEL Assay
In Vivo Imaging
EMSA
Cell Fractionation
Immunoassay
In Vitro Transcription
ChIP-Seq
Northern Blot
3D Imaging
RNAi
ELISPOT
PCR Cloning
Particle Analysis
Southern Blot
Protein Modification
DNA Analysis
Expression
Cell-Based Assay
Gene Editing
In Vivo Testing
Protein Labeling
Other
Species
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Human
Mouse
Rat
Non-Human Primate
Rabbit
Sheep
Hamster
Chicken
Bovine
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Porcine
Horse
Monkey
C. elegans/Worm
Insect
Amoeba/Protozoa
Bacteria/Archaea
Arabidopsis
Yeast/Fungi
Squirrel
Fish
Virus
Other Invertebrate
Xenopus/Amphibian
Drosophila
Goat
Rhesus
Tissue
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Adipose
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Artery
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Blood Vessel
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Brain
Breast
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Cerebral Cortex
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Cornea
Coronary Artery
Dermis
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Embryo
Endometrium
Epidermis
Eye
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Hair
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Hippocampus
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In Vivo
Intestine
Kidney
Large Intestine
Liver
Lung
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Mammary Gland
Mouth
Muscle
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Pancreas
Peripheral Blood
Peritoneum
Placenta
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Rectum
Retina
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Spinal Cord
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Testis
Thymus
Thyroid Gland
Tonsil
Trachea
Umbilical Cord
Umbilical Vein
Uterus
Vein
Cell Type
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Adipocyte
Astrocyte
B Cell
CD8+ T Cell
Competent Cells
Dendritic Cell
Embryonic Stem Cell
Endothelial Cell
Eosinophil
Epithelial Cell
Fibroblast
Glial Cell
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Helper T Cell
Hematopoietic Stem Cell
Hepatocyte
HUVEC
Induced Pluripotent Stem Cell
Keratinocyte
Leukocytes
Lymphocyte
Macrophage
Mesenchymal Stem Cell
Microglia
Microvascular Endothelial Cell
Monocyte
Myoblasts
Myocyte
Neuron
Neutrophil
NK Cell
NKT Cell
Oligodendrocytes
Osteoclast
PBMC
Platelets
Progenitor Cell
Red Blood Cell
Regulatory T Cells
Skeletal Muscle Cell
Smooth Muscle Cell
Splenocyte
Stem Cell
Stromal Cell
Synoviocyte
T Cell
Thymocytes
Tumor Cells
Cell Line
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HeLa
PC3 (PC-3)
HEK293
HEK293T (HEK-293T)
CHO
Jurkat
3T3
Caco-2
HepG2
THP-1
U2OS
PC12 (PC-12)
HL60 (HL-60)
A375 (A-375)
MOLT4 (MOLT-4)
MCF-7
A431
MDA-MB-231
A549
N2a
Daudi
HCT-116
Raji
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