Millipore Sigma
KiCqStart® One-Step Probe RT-qPCR ReadyMix™, Low ROX™
KCQS08
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Our group focuses on the utilization of CD8 T-cells as means of cancer treatment. For our experimental purposes and the development of such TCRs, we often need to screen primary cells and cell lines for our target of interested by qPCR.
qPCR
RNA
The probes should be tested for genomic DNA detection.
This kit combined with Taqman probes allows us to detect and quantify gene expression in a quick and efficient way.
N/A
Works well, no cDNA synthesis step is required.
Expensive
Although the kit is a bit expensive, the time and hassle it saves from using an one-step protocol is worth the price difference and saves a lot of laboratory effort.