The Good
Strong fluorescence, easy to use, cells are fixable, easily combinable with green dyes.
The Bad
Rather fast photobleaching, resolution lower in the far red spectra.
The Bottom Line
Very easy to use reagent. Specific, strong fluorescence. Fixable with methanol, enabling costaining of extra and even intracellular targets. Even the architecture and mitochondrial networsk were detectable at a 63x magnification. It is also possible to combine Deep Red with another staining using green fluorescence (such as Alexa 488) and simultaneously record the fluorescence sinde we observed no "bleeding through" in the other channel when not using DAPI. You need very low laser power, however, be aware of faster photobleaching when using this dye in comparison to organic dyes.