Good Beads for Magnetic Isolation

UiO
Immunology
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Miltenyi Biotec

Product Name:

CD45RO MicroBeads

Catalog Number:

130-046-001

We are an immunotherapy focused group, with interest in CD8 T-cells and their TCRs. For our experimental purposes, we routinely require naïve T-cells from healthy blood donors. We are using this beads to perform magnetic isolation of the naïve population, using the Automacs Pro Separator.

Experimental Design and Results Summary

Application

Magnetic Isolation

Starting Material

N/A

Tips

If many cells, it's best to split the sample in 2 separate tubes.

Results Summary

PBMCs were isolated from blood donors and naïve T-cells were isolated with the help of these beads (alongside CD8 and CD57).

DOI or PMID #

N/A

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Summary

The Good

Easy to use, works wells, reliable between batches.

The Bad

Very expensive and the expiry date is not that long.

The Bottom Line

These beads are performing well and in conjunction with the Auto-Macs the isolation doesn't take that long.

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