Millipore Sigma (Merck)
Monoclonal Anti-Glial Fibrillary Acidic Protein (GFAP) antibody produced in mouse.
G3893
N/A
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I have used this antibody to detect astrocyte among a number of neurons within the mouse brain. It has worked very well in mouse sample.
Immunofluorescence
Mouse brain
Incubation was done with Ab diluted to 1:400 for 16h at 4ºC
BSA 4% in PBS
Incubation was done with 2nd Ab diluted to 1:1,000 for 3h at room temperature
None
Fluorescence (AF 488)
In all cases reactivity is quite good at 1:400 dilution, especially if you need to do morphological analysis. However, if one wants to count astrocytes I am pretty sure this antibody fits well at 1:1,000 dilution. Importantly, this antibody does not have any reactivity in animal cortex due to this protein has a very low expression in physiological situation.
10.3389/fncel.2022.933874
This antibody also works on rat samples.
Great antibody.
I have experienced no issues with this product.
The right choice for detecting astrocytes where there is GFAP expression.