Good NF-kB p65 Antibody

November 23, 2022

University of Texas Medical Branch Galveston

Overall

Quality of Results

Ease-of-Optimization

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Company:

Cell Signaling Technology

Product Name:

NF-κB p65 (C22B4) Rabbit mAb

Catalog Number:

#4764

Clone Number:

N/A

Currently working with pregnancy related cells and doing protein analysis of different markers upon different treatments.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Fetal membrane cells

Primary Incubation

1:1000 dilution, overnight, 4 degree

Blocking Agent

5% nonfat milk in 1× Tris-buffered saline-Tween 20 (TBS-T)

Secondary Incubation

1:10000, 2 hours

Tertiary Incubation

None

Detection

ECL WB; Bio-Rad Image Lab 6.0 software.

Results Summary

This antibody has a 65kDa as MW, and shows a good band in this dilution in the samples that I've been working with.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Good quality.

The Bad

None

The Bottom Line

Highly recommend, has a good signal.

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