Good F4/80 Antibody for IF

November 29, 2022

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

Brilliant Violet 421™ anti-mouse F4/80 Antibody

Catalog Number:

123137

Clone Number:

BM8

Info:Supplier Page View Product Specs

Browse Similar Products: F4/80 Antibodies

I am interested to study myeloid cells in steady state and inflammation. As a positive control for F4/80 I stained the small intestine.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Cryosection of murine small intestine

Primary Incubation

1:100 overnight at RT

Blocking Agent

1% BSA, 1% FCS, 0.3% Triton X, 2% mouse serum, 2% rat serum

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

Image was acquired with Zeiss LSM780 confocal microscope.

Results Summary

Excellent staining of F480+ Macrophages (red) in the lamina propria of the small intestine, Actin (Phalloidin) in green.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Very good staining of macrophages.

The Bad

None

The Bottom Line

Highly recommended to stain Macrophages in mouse crysections.

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