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Seamless Experience With Azenta
(1)
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August 18, 2023
I need to make knockouts in bacteria by swapping the gene of interest with an antibiotic resistance marker. I have used Azenta for verifying...
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Great Marker for Lymphatic Vessels From Novus Biologicals
(1)
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Novus Biologicals, a Bio-Techne Brand
August 18, 2023
Our research focuses on myeloid cell pathology in neurodegenerative diseases. The specific goal of this experiment was to evaluate ...
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Straightforward and Easy-to-Follow IFN Gamma ELISA Kit From Thermo Fisher
(1)
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Thermo Fisher Scientific
August 18, 2023
I'm usually hesitant to order uncoated ELISA kits because of the presumed inconvenience and variability from lot to lot but I had to because...
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PE Anti-Human CD127 Flow Cytometry Antibody From Miltenyi Biotec
(1)
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Miltenyi Biotec
August 17, 2023
I wanted to stain the expression of CD127 on the surface of peripheral NK cells. Therefore, I stained human PBMCs in serial dilutions from 1...
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APC Anti-Human NKG2C Flow Cytometry Antibody From Miltenyi Biotec
(1)
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Miltenyi Biotec
August 17, 2023
I wanted to stain the expression of NKG2C on the surface of peripheral adaptive NK cells. Therefore, I stained human PBMCs in serial ...
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Excellent AF700 Anti-Human CD7 Antibody From BioLegend
(1)
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BioLegend
August 17, 2023
I wanted to stain the expression of CD7 on the surface of peripheral ILCs for sorting. Therefore I stained human PBMCs in serial dilutions ...
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PE/Vio770 Anti-Human CD1c Flow Cytometry Antibody From Miltenyi Biotec
(1)
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Miltenyi Biotec
August 17, 2023
I wanted to stain the expression of CD1c on the surface of peripheral cDC2s. Therefore, I stained human PBMCs in serial dilutions from 1:25,...
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BV510 CD69 Flow Cytometry Antibody From BioLegend
(1)
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BioLegend
August 17, 2023
To test this antibody, I stained PBMCs for 30 minutes at room temperature in the dark at serial dilutions of 1:25, 1:50, 1:100, 1:200, and 1...
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Good Beta-Tubulin Antibody From Abcam
(1)
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Abcam
August 16, 2023
I use beta-tubulin antibody as a loading control for protein gels as it does change under various stress conditions.
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Anti-Human PD-1 BUV737 From BD Biosciences Works Well
(1)
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August 16, 2023
I used it for phenotyping human PBMC by flow cytometry to study inflammatory disease. 5 lase Cytek Aurora was used and it works great.
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Great Marker for Lymphatic Vessels From Abcam
(1)
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Abcam
August 16, 2023
The specific goal of experiments using this antibody was to assess alterations in the lymphatics in disease status. This antibody worked ...
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Must Have Imaging System for Cell Culture Labs From Invitrogen
(1)
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Thermo Fisher Scientific
August 16, 2023
Transient transfections, cell staining, and stable cell line preparation are frequent and common experiments that I perform while using cell...
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A Great Blood Vessel Endothelium Marker From Novus Biologicals
(1)
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Novus Biologicals, a Bio-Techne Brand
August 16, 2023
Our research focuses on understanding the role played by glia-vascular interaction in neurodegenerative diseases. The specific goal of this ...
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Fluorescent Dye From Thermo Fisher for Mitochondria Membrane Potential
(1)
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Thermo Fisher Scientific
August 16, 2023
TMRM is a cell-permeant, cationic, red fluorescent dye that is readily sequestered by active mitochondria and can be detected by either ...
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Good Beta-Actin Antibody From Abcam
(1)
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Abcam
August 16, 2023
I use actin antibody as a loading control for my Western blots. It especially works great for insoluble protein fractions.
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Convenient DNA Gel Extraction Kit From Qiagen
(1)
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QIAGEN - Sample to Insight
August 16, 2023
This kit is very useful for extraction of DNA from agarose gels especially if you have non-specific PCR-amplified DNA bands.
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Best Transfection Reagent From Thermo Fisher
(1)
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Thermo Fisher Scientific
August 16, 2023
I frequently perform cell culture transfections of my protein of interest tagged with fluorescent proteins for either stable cell line ...
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Good and Cheap Flag Antibody From Proteintech
(1)
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Proteintech Group Inc
August 15, 2023
The antibody works well for detecting flag-tagged proteins in Western blot. It can also be used for immunoprecipitation.
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Anti-Mouse IFN-γ Antibody for Flow Cytometric Quantification
(1)
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BioLegend
August 15, 2023
I study tumor immune microenvironment in preclinical primary and metastatic models of colorectal cancer. My research involves extensive ...
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Activation Cocktail for Cytokine Quantification by Flow Cytometry
(1)
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BioLegend
August 15, 2023
My research area includes immune phenotyping of primary or metastatic tumors. I often do ex vivo stimulation of T cells sorted from the ...
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Good and Cheap MYC Tag Antibody From Proteintech
(1)
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Proteintech Group Inc
August 15, 2023
The antibody works great for detecting myc-tagged proteins in Western blot. It gives a good signal even in higher dilutions.
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Beads Based Compensation Controls for Flow Cytometry
(1)
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Thermo Fisher Scientific
August 15, 2023
My research area includes immune phenotyping of primary or metastatic tumors. I often do a complex flow cytometry panel for my analysis. The...
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Anti-Mouse B-Cell Antibody in UV Channel From BD Biosciences
(1)
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August 15, 2023
I study tumor immune microenvironment in preclinical primary and metastatic models of colorectal cancer. My research involves extensive ...
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Viability Dye in UV Channel From BioLegend
(1)
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BioLegend
August 15, 2023
I used this viability kit for my invitro proliferation assays for organoids and immune phenotyping of my tumor microenvironment assays. It ...
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BrdU-PE staining kit for Proliferation
(1)
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Thermo Fisher Scientific
August 15, 2023
I used this proliferation kit for my in vitro proliferation assays for organoids and ex vivo T cell proliferation quantification. It gives a...
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Highly Recommended Genomic DNA Isolation Kit From New England BioLabs
(1)
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New England Biolabs
August 14, 2023
In order to clone bacteria genes, I must extract genomic DNA (gDNA) using this kit, which is more efficient than the traditional ...
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Fantastic enzyme to solubilize cell walls of bacteria
(1)
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MilliporeSigma
August 14, 2023
I work extensively with cell wall hydrolases (enzymes) of bacteria. To check the enzymatic activity, I have to solubilize the intact cell ...
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Test strips to determine Minimum Inhibitory Concentration (MIC) of bacteria
(1)
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August 14, 2023
I want to investigate the genetic connection between cell wall hydrolase knockouts of bacteria and cell wall-acting antibiotic vancomycin. ...
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Streamlined Plasmid DNA Purification Kit From Zymo Research
(1)
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Zymo Research
August 14, 2023
I do a lot of clonings in my lab as a part of making gene knockouts in bacteria. In this process, I have to make a lot of plasmid preps and ...
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Reliable E. coli Competent Cells From New England BioLabs
(1)
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New England Biolabs
August 14, 2023
I have constructed a relatively large shuttle vector that can be propagated in both Gram-positive (E. faecium) and Gram-negative bacteria (E...
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Tritiated-Meso-Diaminopimelic Acid From Moravek, a Proxy to Determine Peptidoglycan Incorporation in Bacteria
(1)
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Moravek Biochemicals
August 14, 2023
I have used 3H-mDAP as a proxy, to determine the amount of cell wall incorporation in a cell wall hydrolase mutant.
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User Friendly Cell Counter From Logos Biosystems
(1)
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Logos Biosystems
August 14, 2023
My research is focused on developing a novel Pyk2 inhibitor that will inhibit Invadopodia formation thus, inhibiting metastasis formation in...
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Reliable Kit From ThermoFisher for Bacterial Live-Dead Cell Staining
(1)
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Thermo Fisher Scientific
August 14, 2023
I made a cell wall hydrolase knockout in E. coli and I observed decreased growth in a growth curve. To investigate whether the effect is ...
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Excellent Anti-MTDH From Invitrogen
(1)
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Thermo Fisher Scientific
August 13, 2023
I used this antibody to detect the expression of MTDH in my WB assays using breast cancer cell line MCF7. It detected a very clear band and ...
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Anti-APOA1 Exosome Marker From Thermo Fisher
(1)
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Thermo Fisher Scientific
August 13, 2023
I used this antibody to check the expression of APOA1, which is a marker for exosomes. I confirmed positive expression in all isolated ...
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Good Anti-TSG101 Exosome Marker From Santa Cruz
(1)
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Santa Cruz Biotechnology, Inc.
August 13, 2023
I used this antibody to check the expression of TSG101, which is a marker for exosomes. I confirmed positive expression in all isolated ...
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Best Lentiviral Transduction Reagent Available on the Market From SIRION Biotech
(1)
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August 13, 2023
This reagent was the best that I worked with optimizing my transduction of primary T-cells. Compared to polybrene, this was both less ...
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Reliable Alpha-Tubulin Antibody From CST
(1)
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Cell Signaling Technology
August 13, 2023
I used this antibody for my Western blots and used it to detect the expression of internal control alpha-tubulin. It gave me a very clear ...
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Good Choice of TRPV6 Antibody From Alomone Labs
(1)
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Alomone Labs, Ltd.
August 13, 2023
I used this antibody to check the expression of TRPV6 calcium channel expression in cancer cell lines with siRNA control or siRNA that ...
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BUV496 Mouse Anti-Human CD25 From BD Biosciences Works Well
(1)
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BD Biosciences
August 13, 2023
I worked on inflammatory disease using human samples like PBMC and used this antibody as an activated T-cell marker. I used it for ...
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Good Mineral Supplement For Bacterial Culture From ATCC
(1)
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August 12, 2023
We previously found a small metabolite that boosts energy expenditure in mice. Interestingly this metabolite is synthesized from the gut ...
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Great Thyroid Stimulating Hormone ELISA Kit From Abcam
(1)
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Abcam
August 12, 2023
We are screening an array of endogenous metabolites found in human serum for bioactivity. Thyroid stimulating hormone and thyroid hormone ...
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Anti-CD81 Exosome Marker From Santa Cruz
(1)
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Santa Cruz Biotechnology, Inc.
August 12, 2023
I used this antibody to check the expression of CD81, which is a marker for exosomes. I confirmed positive expression in all isolated ...
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LipidSpot 610 From Biotium
(1)
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Biotium, Inc.
August 12, 2023
I used this to stain lipids in one of my adipogenic induction experiments using human mammary pre-adipocytes. After treatment with ...
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Reliable Ki67 Antibody From CST
(1)
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Cell Signaling Technology
August 12, 2023
I used this antibody to stain proliferating cells for my MDAMB231 xenografted tumors, and it showed a very good signal on my tissue sections...
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Good, But Pricey Antibody From R&D Systems
(1)
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R&D Systems, a Bio-Techne Brand
August 12, 2023
We performed in vitro T helper polarization assays using human CD4+ T cells. We utilized this anti-human IL-12 antibody, along with an anti-...
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Great BCAA Quantification Kit From Abcam
(1)
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Abcam
August 12, 2023
We previously pharmacologically identified a small metabolite that regulates energy expenditure in mice. In this study, we want to explore ...
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Good GAPDH Antibody From CST
(1)
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Cell Signaling Technology
August 12, 2023
I used this antibody as an internal control for my western blots, to verify the effects of a set of shRNAs. The antibody worked really well ...
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E. coli Polar Lipid Extract From Avanti is Consistent in Quality
(1)
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Avanti Polar Lipids Inc.
August 12, 2023
We have been trying to develop a mass spectrometry pipeline for quantifying hundreds of lipids in the same run. To do so, we need a library ...
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Great Cholesterol Assay Kit From Abcam
(1)
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Abcam
August 12, 2023
We previously pharmacologically identified a small metabolite that regulates energy expenditure in mice. In this study, we want to explore ...
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