Excellent Anti-MTDH From Invitrogen

August 13, 2023

Surgery
University of Texas Southwestern Medical Center
Research Scientist

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
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Company:

Invitrogen/Thermo Fisher

Product Name:

Metadherin Polyclonal Antibody

Catalog Number:

40-6500

Clone Number:

N/A

I used this antibody to detect the expression of MTDH in my WB assays using breast cancer cell line MCF7. It detected a very clear band and showed decreased expression in a microRNA X transfected sample.

Experimental Design and Results Summary

Applications

Western blot

Sample

MCF7 cells

Primary Incubation

1:250, 4 degree, O/N

Blocking Agent

5% non-fat milk

Secondary Incubation

1:20000, 1h at RT

Tertiary Incubation

None

Detection

Amersham ECL and picture taken using Chemidoc.

Results Summary

I used this antibody to detect the expression of MTDH in my WB assays using breast cancer cell line MCF7. It detected a very clear band and showed decreased expression in a microRNA X transfected sample.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Clear band,and no background.

The Bad

N/A

The Bottom Line

A good reliable antibody for MTDH.

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