R&D Systems
Human IL-12 Antibody
AF-219-NA
Polyclonal
Info: Supplier Page View Product Specs
Browse Similar Products: Anti-IL 12 Antibody Products
We performed in vitro T helper polarization assays using human CD4+ T cells. We utilized this anti-human IL-12 antibody, along with an anti-human IFNg antibody, to prevent unwanted Th1 polarization in our Th2 culture conditions. Our Th2 polarizations were a success, with minimal contamination with unwanted Th1 cells.
Block/Neutralize/Inhibit
Human naive CD4+ T cells
5 ug/mL during a 5 day culture
N/A
IFNg AlphaLISA
This anti-human IL-12 antibody was able to prevent unwanted Th1 polarization in Th2-promoting conditions. We confirmed the absence of Th1 contamination in our Th2 cultures using flow cytometry and IFNg AlphaLISA assays. The Th2 cultures produced IL-5 but not IFNg, as expected.
Prevents Th1 contamination in Th2 cultures.
Expensive.
Expensive, but great neutralizing antibody for human IL-12.