A Great Blood Vessel Endothelium Marker From Novus Biologicals

Neuroscience
Wisconsin University
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

Novus Biologicals

Product Name:

CD31/PECAM-1 Antibody

Catalog Number:

NB600-562

Clone Number:

JC/70A

Our research focuses on understanding the role played by glia-vascular interaction in neurodegenerative diseases. The specific goal of this experiment was to evaluate vasculature in the course of disease progression. This antibody demonstrated excellent staining for blood vessels in the brain.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Mouse brain

Primary Incubation

1:50 at 4 degrees celsius for 2 days.

Blocking Agent

5% normal donkey serum

Secondary Incubation

Alexa fluor anti-mouse IgG at 1:500 for 2hr at room temp.

Tertiary Incubation

N/A

Detection

Fluorescence

Results Summary

Robust immunofluorescence signals from the antibody labelling for CD31 positive vasculatures endothelium were detected in mouse brain sections.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Reliable robust signals.

The Bad

None.

The Bottom Line

This antibody works well for mouse and we would buy it again.

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