Great Marker for Lymphatic Vessels From Abcam

Neuroscience
Wisconsin University
Postdoctoral Researcher

Overall

Quality of Results

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Company:

Abcam

Product Name:

Recombinant Anti-PROX1 antibody [EPR19273] (ab199359)

Catalog Number:

ab199359

Clone Number:

NA

The specific goal of experiments using this antibody was to assess alterations in the lymphatics in disease status. This antibody worked great as a specific marker for lymphatic vessels.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Mouse lymph node

Primary Incubation

1:200 at 4 degrees celsius for overnight.

Blocking Agent

5% normal donkey serum

Secondary Incubation

Alexa fluor anti-mouse IgG at 1:500 for 1hr at room temp

Tertiary Incubation

N/A

Detection

Fluorescence

Results Summary

This antibody worked well as a tool to assess lymphatic vessels. PROX1 is known as a specific transcription factor for lymphatic vascular endothelia. This antibody showed little background but high specificity when used for tissue sections.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

High specificity.

The Bad

None

The Bottom Line

This is a great PROX1 antibody providing reproducible staining results.

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