Reliable Ki67 Antibody From CST

Surgery
University of Texas Southwestern Medical Center
Research Scientist

Overall

Quality of Results

Ease-of-Optimization

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Company:

Cell Signaling Technology

Product Name:

Ki67 Antibody

Catalog Number:

#9027

Clone Number:

N/A

I used this antibody to stain proliferating cells for my MDAMB231 xenografted tumors, and it showed a very good signal on my tissue sections.

Experimental Design and Results Summary

Applications

Immunohistochemistry

Sample

MDAMB231 Tumors

Primary Incubation

1:500, 4 degrees, O/N

Blocking Agent

2.5% horse serum

Secondary Incubation

1:1000, 1h at RT

Tertiary Incubation

None

Detection

DAB kit form Dako

Results Summary

I used this antibody to stain proliferating cells for my MDAMB231 xenografted tumors and it showed a very good signal on my tissue sections. Around 80% of the cancer cells (demonstrated by large nucleus compared to normal cells) are stained positive.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Clear nuclear staining.

The Bad

N/A

The Bottom Line

Reliable staining of proliferating cells on tumor sections.

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