Cell Signaling Technology
anti-GAPDH
#2118
N/A
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I used this antibody as an internal control for my western blots, to verify the effects of a set of shRNAs. The antibody worked really well and gave a clear band at around 37 KDa.
Western blot
Human Colorectal cancer cell line HCT116
1:5000, 4 degrees, O/N
5% non-fat milk
1:20000, 1h at RT
None
Amersham ECL and picture taken using Chemidoc
It showed a very clear band at 37 KDa without background and the signal is not affected by shRNAs targeting an irrelevant gene of interest.
NA
Clear band, and no background.
Very reliable antibody that give clear bands.