Good and Cheap MYC Tag Antibody From Proteintech

Department of Pathology
Stanford University
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
Write a Review

Company:

Proteintech

Product Name:

MYC tag Polyclonal Antibody

Catalog Number:

16286-1-AP

Clone Number:

N/A

The antibody works great for detecting myc-tagged proteins in Western blot. It gives a good signal even in higher dilutions.

Experimental Design and Results Summary

Applications

Western blot

Sample

Protein lysate from transfected Hela cells

Primary Incubation

1:5000 in 0.5% milk incubated overnight at 4 degrees C.

Blocking Agent

5% Milk in Tris-buffered Saline Tween-20

Secondary Incubation

1:2000 HRP-conjugated anti-Rabbit IgG, 1 hour at RT

Tertiary Incubation

N/A

Detection

ECL detection developed on X-ray film.

Results Summary

The antibody gives a clean band for the transfected myc-tagged protein. It does not give any background.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Good and clean signal,and can be used at higher dilutions.

The Bad

None.

The Bottom Line

Cheap and good antibody. Highly recommended.

Share your experience with other scientists. Write a Review! »

Join the discussion