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Date Published
PowerPac Basic Power Supply From Bio-Rad
(1)
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Bio-Rad
July 29, 2023
We are interested in understanding how proteins respond to stress in mammalian cells. We routinely run SDS-PAGE gels to detect proteins ...
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Deoxyribonuclease I, Alexa Fluor™ 488 Conjugate From Invitrogen
(1)
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Thermo Fisher Scientific
July 29, 2023
In cells, soluble G-actin is maintained in a dynamic equilibrium with F-actin and the extent of actin polymerization and depolymerization ...
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B-PER™ Complete Bacterial Protein Extraction Reagent
(1)
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Thermo Fisher Scientific
July 29, 2023
As a researcher working with purified proteins from bacteria, the B-PER™ Complete Bacterial Protein Extraction Reagent has become an ...
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Cheap and Simple On-Column DNase Kit
(1)
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July 28, 2023
Total RNA was isolated from lysed cells and extracellular vesicles for qPCR and miRNA sequencing. Column-based isolation was performed, ...
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Great Antibody for GM130 detection From BD Biosciences
(1)
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BD Biosciences
July 28, 2023
We use this monoclonal mouse GM130 antibody frequently in our immunofluorescence staining to stain for cis-Golgi cisternae. Staining results...
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Excellent FOXP3 Antibody for Flow Cytometry From BioLegend
(1)
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BioLegend
July 28, 2023
Great staining of FOXP3 in mouse immune cells from the intestines. Very clear signal and great separation of positive population.
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Excellent CD8a Antibody From BioLegend
(1)
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BioLegend
July 28, 2023
Very strong and specific staining of CD8a in mouse T-cells. Great separation of positive and negative populations.
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Excellent Staining With CD4 Antibody From BioLegend
(1)
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BioLegend
July 28, 2023
Great separation of the CD4 positive population from the negative when intestinal cells are stained with this antibody.
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Very Clear Staining With Cyclin E1 Rabbit mAb From Cell Signaling
(1)
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Cell Signaling Technology
July 28, 2023
We were testing the effects of a knockout/knockdown of an E3-ubiquitin ligase known to interact with CCNE1.
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Alexa Fluor™ 546 Phalloidin From Invitrogen for Labeling F-Actin in Fixed Cells
(1)
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Thermo Fisher Scientific
July 28, 2023
To explore the possible reorganization of the actin cytoskeleton as a consequence of cholesterol depletion, it is crucial to quantitatively ...
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Effective Ultrafilter for Extracellular Vesicle/Exosome Concentration and Staining
(1)
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MilliporeSigma
July 28, 2023
Extracellular vesicles (EVs) isolated from cell culture media and resuspended in buffer were concentrated down to small volumes using 0.5ml ...
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Great CD4 Antibody From BD Biosciences
(1)
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BD Biosciences
July 28, 2023
Great staining of the CD4 in mouse immune cells. Very clear separation of positive and negative populations allowing specific gating of CD4-...
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Handy Filter Sterilization of Culture Media From Millipore Sigma
(1)
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MilliporeSigma
July 26, 2023
Cell culture media prepared with non-sterile reagents can be filtered through the Steriflip unit for filter sterilization before application...
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Positive Control Stimulus for T-cell Activation
(1)
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July 26, 2023
T-cell activation generally requires specific activation of the T-cell receptor. Antigen-independent activation can be achieved using PMA to...
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Excellent IL-7A Antibody From BD Biosciences
(1)
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BD Biosciences
July 26, 2023
Great staining of intracellular IL-7A in mouse immune cells. The separation of the positive population is decent.
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Antibody With Great Staining for Flow From BioLegend
(1)
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BioLegend
July 26, 2023
Great staining of mouse CD90 for flow cytometry. Very specific and strong signal giving good separation of positive and negative populations...
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Simple Centrifugal Filter Unit for Ultrafiltration of Culture Media From Millipore Sigma
(1)
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MilliporeSigma
July 26, 2023
Cells were cultured in monolayers for extracellular vesicle (EV) isolation. Centrifugal filter units (100kDa size cutoff) were used to ...
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Gel Filtration Molecular Weight Calibration Kit From Cytiva
(1)
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Cytiva Life Sciences
July 25, 2023
I am interested in understanding the molecular mechanism of the Hedgehog signal transduction pathway. We routinely purify proteins for ...
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Coupled Transcription/Translation Kit From Promega
(1)
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Promega
July 25, 2023
Hedgehog signaling plays a critical role in embryonic development. It also controls adult tissue homeostasis and deregulation of this ...
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Genome Editing Efficiency Determination by T7 Endonuclease I (T7EI) Mismatch Cleavage Assay
(1)
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Integrated DNA Technologies
July 25, 2023
I routinely use CRISPR/Cas9-based methodology to generate gene knockouts in cell lines. As a first step, I do pooled gene knockout using the...
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Efficient RNeasy Mini Kit for Fast and Simple RNA Purification
(1)
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July 25, 2023
RNeasy Mini Kit allows the purification of RNA from small amounts of starting material (e.g., animal or yeast cells, mice tissues), useful ...
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Gamma H2A.X Antibody for Western blot analysis From Abcam
(1)
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Abcam
July 25, 2023
The cell lysate was meticulously prepared from Human mesenchymal stem cells by established laboratory procedures. Subsequently, 25 µg of ...
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Great Equipment Used for the Production of Moss-Derived Biopharmaceuticals
(1)
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CYTENA
July 25, 2023
With the single-cell dispenser UP.SIGHT by Cytena, we developed a new method of strain development in our company to automate the whole ...
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Substrate for Alkaline Phosphatase Assay From Sigma Aldrich
(1)
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MilliporeSigma
July 25, 2023
Hedgehog signaling plays a critical role in embryonic development. It also controls adult tissue homeostasis and deregulation of this ...
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Preferred Dissociated Reagent for Feeder-Free Stem Cell Culture
(1)
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July 25, 2023
Both human iPSC and human ESC were cultured in feeder-free conditions on 1:100 matrigel-coated culture plates. ReLeSR reagent was ...
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Good Assay Kit for LDLR binding assay
(1)
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BPS Bioscience, Inc.
July 24, 2023
This kit is very useful for the drug acting on hyperlipidemia. This helps to identify the binding of PCSK9 to LDLR.
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RNA Synthesis Kit From NEB
(1)
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New England Biolabs
July 23, 2023
The WNT pathway is essential for embryonic development and adult tissue homeostasis. Deregulation of this pathway results in cancers of the ...
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High Binding Capacity Streptavidin Resin for Capturing Biotinylated Proteins
(1)
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July 23, 2023
Hedgehog signaling plays a critical role in embryonic development. It also controls adult tissue homeostasis and deregulation of this ...
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Nice Anti-Gamma H2A.X Antibody
(1)
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Abcam
July 23, 2023
Gamma H2A.X is a central component of numerous signaling pathways in response to DNA double-strand breaks. I used this antibody to stain the...
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Nice Fluorophore Option for Pan T-cell Marker From Invitrogen
(1)
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Thermo Fisher Scientific
July 23, 2023
PBMC-derived T-cells were activated with CD3/CD28 magnetic beads and IL-2 for 3 days and FACS stained with this antibody according to the ...
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Di-tert-butyl dicarbonate for Boc Protection
(1)
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MilliporeSigma
July 23, 2023
The aim was to protect just one side of a diamine to be subsequently able to link it to a second scaffold.
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ECL Substrate - Femto From Thermo Scientific
(1)
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Thermo Fisher Scientific
July 23, 2023
The WNT pathway is essential for embryonic development and adult tissue homeostasis. De-regulation of this pathway results in cancers of the...
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Good ECL Western Blotting Reagent From ThermoFisher
(1)
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Thermo Fisher Scientific
July 23, 2023
The WNT pathway is essential for embryonic development and adult tissue homeostasis. De-regulation of this pathway results in cancers of the...
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Trifluoroacetic Acid for Boc Deprotection From Oakwood Chemical
(1)
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Oakwood Products , Inc.
July 23, 2023
The goal of the work was to remove the Boc protective group from an amide to go further in the synthesis, following a coupling reaction to ...
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Convenient and Hassle Free Staining of Dead Cells by Flow Cytometry
(1)
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Thermo Fisher Scientific
July 23, 2023
Very easy to use viability dye, just resuspend your cells in the FACS buffer with the viability dye in it during the last step of staining ...
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Effective Reducing Agent From Sigma Aldrich
(1)
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MilliporeSigma
July 23, 2023
This product was used to reduce ester to aldehydes avoiding the risk to get the corresponding alcohol
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ECL Substrate - Pico PLUS From Thermo Scientific
(1)
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Thermo Fisher Scientific
July 23, 2023
The WNT pathway is essential for embryonic development and adult tissue homeostasis. De-regulation of this pathway results in cancers of the...
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Nice CD4
+
CD25
+
Regulatory T Cell Isolation Kit
(1)
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Miltenyi Biotec
July 23, 2023
I used this kit in a project to study the release of extracellular vesicles from T regular cells. This kit worked well to isolate CD4
+
CD25
+
...
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siRNA Transfection Reagent for Cell Lines From ThermoFisher
(1)
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July 23, 2023
The WNT pathway is essential for embryonic development and adult tissue homeostasis. Deregulation of this pathway results in cancers of the ...
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Easy to Use Fixable Viability Dye
(1)
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Thermo Fisher Scientific
July 23, 2023
1 million PBMC-derived T-cells were resuspended in PBS and stained with 1 µl of this dye at RT for 10-15 minutes. Cells were washed with ...
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Great Phosphatase Inhibitor Cocktail Solution From GenDEPOT
(1)
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July 23, 2023
We have been using this phosphatase inhibitor cocktail solution in Western blot to prevent proteins from dephosphorylation for several years...
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Synthesis of Schiff Base
(1)
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MilliporeSigma
July 23, 2023
We synthesized a Schiff base using an amine and an aldehyde. This product will be then linked to a larger scaffold exploiting the imine ...
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Trifluoroacetic Acid From Thermo Scientific For Boc Deprotection
(1)
Write a Review
July 23, 2023
We need to deprotect a Boc amine to be able then to link it to a carboxylic acid and form a new peptide bond.
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Nice Anti-mouse CD45 Antibody For Flow Cytometry From BioLegend
(1)
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BioLegend
July 23, 2023
We have used this CD45 antibody in several of our projects. It worked well for staining leukocytes in single cells isolated from the spleen ...
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Cell Surface Labeling Reagent From Thermo Fisher
(1)
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Thermo Fisher Scientific
July 23, 2023
Hedgehog signaling plays a critical role in embryonic development. It also controls adult tissue homeostasis and deregulation of this ...
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Excellent MHC-II Antibody From BD Biosciences
(1)
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BD Biosciences
July 22, 2023
The staining is extremely bright. I'd recommend using much less of this antibody compared to others, especially when running flow cytometry.
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Great Antibody for Immunofluorescence From Millipore Sigma
(1)
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MilliporeSigma
July 22, 2023
Blood vessels in the brain of mice are identified by staining for basement membrane glycoprotein laminin. Regions, where the basement ...
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Great T-Cell Antibody for Flow Cytometry From BioLegend
(1)
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BioLegend
July 22, 2023
CD8+ T-cell infiltration into the brain during viral respiratory infection in mice is assessed by flow cytometry.
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Great Viability Dye for Flow Cytometry From BioLegend
(1)
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BioLegend
July 22, 2023
Inflammation in brain endothelial cells is measured during viral respiratory infection in mice. Zombie Green viability dye was used to ...
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Great Antibody for VCAM-1 Staining From BioLegend
(1)
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BioLegend
July 22, 2023
Expression of VCAM-1 on mouse brain endothelial cells during viral respiratory infection was measured by flow cytometry.
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