Viability Dye in UV Channel From BioLegend

August 15, 2023

The Salk Institute of Biological Studies
NOMIS CENTER FOR IMMUNOBIOLOGY AND MICROBIAL PATHOGENESIS
Postdoctoral Researcher

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Quality of Results

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Company:

BioLegend

Product Name:

Zombie UV fixable viability kit

Catalog Number:

423107

I used this viability kit for my invitro proliferation assays for organoids and immune phenotyping of my tumor microenvironment assays. It is a perfect viability kit that discriminates dead cell populations from live cells.

Experimental Design and Results Summary

Application

Discrimination of dead cells from live cells for flow Cytometry experiment.

Starting Material

Cells from culture or tissue.

Tips

This is light sensitive dye so always prepare fresh or 1-2 hours before use.

Results Summary

The following result is obtained from the intestinal organoid culture proliferation experiment. I stained 0.2 million cells harvested from organoids with Zombie UV at 1:1000 dilution for 20 mins on ice and then fixed with 2% of paraformaldehyde for 30 mins on ice. The zombie UV stained and discriminated against the dead population from the culture. The dye is excited by a UV laser and emits in the DAPI channel. So dead population is shown here as DAPI positive contour.

DOI or PMID #

N/A

Image Gallery

Summary

The Good

Works very good even at lower concentration. I used it between 1:500 to 1:1000 dilutions.

The Bad

Light sensitive but it's not a drawback. Use Aluminum foil.

The Bottom Line

The Zombie UV dye is excited by a UV laser and emits in the DAPI channel. So it leaves other important channels open. In complex immune phenotyping experiments this could be very helpful.

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