Straightforward and Easy-to-Follow IFN Gamma ELISA Kit From Thermo Fisher

August 18, 2023

Immunology
George Washington University
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

Invitrogen

Product Name:

IFN gamma Human Uncoated ELISA Kit

Catalog Number:

88-7316-88

Clone Number:

N/A

I'm usually hesitant to order uncoated ELISA kits because of the presumed inconvenience and variability from lot to lot but I had to because the coated version was backordered. However, this worked out much better than I thought. It does require more samples than what I've seen usually asked for (100 uL versus 50 uL) but is otherwise a kit I'd suggest to ELISA users.

Experimental Design and Results Summary

Applications

ELISA

Sample

Cell culture supernatant from immune cells

Primary Incubation

Overnight at 4C

Blocking Agent

ELISA diluent for 1 h at RT.

Secondary Incubation

Samples/standards for 2 h at RT.

Tertiary Incubation

RT for 2h or overnight at 4 degrees C.

Detection

Streptavidin-HRP

Results Summary

This ELISA helped me determine how much soluble IFN gamma is released by my immune cells following contact with protein of interest.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Straightforward and clear instructions.

The Bad

Uncoated, washing steps are a bit tedious (5X).

The Bottom Line

ELISA kit was as simple as most kits go. There weren't any confusing instructions and I had no issues using it for the first time. I'd generally recommend the coated ELISA kits but this will also work just fine.

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