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Rapid and High Performative Protein Desalting Columns
(1)
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June 30, 2023
In our lab, we use Zeba Spin Desalting Columns due to their lower cost and most importantly, because they are a ready-to-use and compatible ...
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Convenient And Ready to Use Medium for Selective Bacterial Culture
(1)
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Teknova
June 30, 2023
In our lab, we use an LB Agar plate with 50µg/ml kanamycin for selective cultivation of E. coli strains that are kanamycin resistant and for...
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Glacial Acetic Acid 100%, Water Free
(1)
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MilliporeSigma
June 30, 2023
We bought the Glacial acetic acid because it is 100% water-free acid, so it is more concentrated. I used the reagent within the ...
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Cuvettes for Medium-Large Quantity Cell Electroporation
(1)
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June 28, 2023
We equipped the OU lab with the Lonza 4D Nucleofector System for the electroporation of different cell types. The system requires dedicated ...
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Choose Correct Phospho-p44/42 (Erk1/2)
(1)
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Cell Signaling Technology
June 27, 2023
MDA-MB-415 cells were treated with 0.3 µM Neratinib for 3 days. The immunoblotting procedure is described below. Lyse cells by adding RIPA...
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ACC1 Antibody for Western Blot
(1)
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Santa Cruz Biotechnology, Inc.
June 27, 2023
To test whether 25-OH cholesterol treatment in human THP-1 cells affects Acetyl-CoA carboxylase (ACC1) expression. The results showed that ...
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True Semi-Dry Transfer for Immunoblot
(1)
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Bio-Rad
June 27, 2023
I loaded 20 ug of protein samples on 4–12% Criterion™ XT Bis-tris protein gel, 12+2 well, 45 µl #3450123. The protein was then transferred ...
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Reagent For Efficient Bcl-2 Inhibition
(1)
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Selleck Chemicals
June 26, 2023
Venetoclax from Sellekchem is a potent Bcl-2 inhibitor. We regularly use it for studying the Bcl-2 protein family network and apoptosis. ...
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Very Good Medium to Sustain T Cell Expansion
(1)
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June 26, 2023
We work mainly with primary T cells (both murine and human) and recently we experienced many problems in finding a proper calf serum to ...
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Excellent Media For Secreted Protein Purification
(1)
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Thermo Fisher Scientific
June 26, 2023
We are developing chimeric, secreted proteins that require mammal/human post-translational modifications. We, therefore, used the suspension...
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Total ERK1/2
(1)
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Cell Signaling Technology
June 26, 2023
MDA-MB-415 cells were treated with 0.3µM Neratinib and RSL3 for 3 days. The immunoblotting procedure is described below. Lyse cells by ...
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Ready to Use Pre-Weighted TAE Powder
(1)
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Dutscher Scientific UK Ltd
June 24, 2023
We perform a lot of agarose electrophoresis that require a certain volume of TAE buffer and reproducibility between preparations. This ...
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Nice Anti-CD8a Antibody
(1)
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Tonbo Biosciences
June 22, 2023
I have been using this Anti-CD8a Antibody in several studies. This antibody worked well in identifying CD8-positive T cells in the single ...
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FAS Antibody Works for Western Blot
(1)
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Santa Cruz Biotechnology, Inc.
June 22, 2023
To test whether 25-OH cholesterol treatment in human THP-1 cell affects fatty acid synthase (FAS) expression. The results showed that 25-OH ...
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Great Anti-Mouse F4/80 Antibody
(1)
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Tonbo Biosciences
June 22, 2023
Anti-F4/80 Antibody recognizes the mouse F4/80, a marker for murine macrophages. I used this Anti-F4/80 Antibody to stain the mice bone ...
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Nice Nitric Oxide Indicators- DAF-FM Diacetate
(1)
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Thermo Fisher Scientific
June 21, 2023
I used this DAF-FM™ diacetate reagent to detect cellular nitric oxide level change, after drug treatment.
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Sensitive SYBR Green Reagent by ABclonal
(1)
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ABclonal Technology
June 20, 2023
The 2X Universal SYBR Green Fast qPCR Mix contains de novel designed universal reference dye, which can realize higher signal resolution and...
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Simple and High Quality RNA Isolation Kit
(1)
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Thermo Fisher Scientific
June 20, 2023
RNA was isolated from kidney podocyte cell cultures grown on different substrates to compare the effect of culture condition on gene ...
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Great Phalloidin for Staining of the Actin Cytoskeleton
(1)
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Thermo Fisher Scientific
June 20, 2023
I have used this Alexa Fluor™ 555 Phalloidin in one of studies to compare cell morphology change after treatment with my drug. I stained the...
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Anti-HA Tag Antibody from Abcam
(1)
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Abcam
June 20, 2023
To identify a HA-tag primary antibody to detect the exogenous expression of the constructed plasmids in OE19 stable cell models, five HA-tag...
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Great IAk Antibody with Strong Staining
(1)
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BioLegend
June 16, 2023
We use this antibody often to identify and characterize different antigen presenting cells from NOD mouse spleen and lymph nodes. It stains ...
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Human Phospho-H2AX ELISA Works Well as I Expected
(1)
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RayBiotech
June 16, 2023
I analyzed EV (extracellular vesicle) samples. I detected Phospho-H2AX (S139) on my samples with high confidence. Positive control ...
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Excellent and Easy to Use Kit for Monitoring Cell Viability
(1)
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Promega
June 16, 2023
The kit provides a homogeneous, fluorescent method for monitoring cell viability. The assay is based on the ability of living cells to ...
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Good CD90.2 BV570 Antibody from BioLegend
(1)
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BioLegend
June 16, 2023
We use this antibody to identify T cells and ILCs from mouse spleen routinely by flow cytometry. It works well to detect those lymphocytes ...
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GAPDH Antibody Worked Well for Western Blot
(1)
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Proteintech Group Inc
June 16, 2023
My research involves studying how D1 dopamine receptor, a member of the GPCR superfamily, can signal from distinct membrane compartments ...
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Anti-HA Tag Antibody Works Well
(1)
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Cell Signaling Technology
June 16, 2023
Used to identify an HA-tag primary antibody to detect the exogenous expression of the constructed plasmids in OE19 stable cell models, five ...
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Reliable Antibody for Human CD3
(1)
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BioLegend
June 16, 2023
This antibody targets the epsilon subunit of CD3. It offers robust signal in the PE channel and can be used in gating for T cells in flow ...
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Flexible Secondary Antibody for Immunofluorescence
(1)
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Thermo Fisher Scientific
June 15, 2023
Epicardial cells were differentiated from human embryonic stem cells and stimulated with four different growth factor conditions. The ...
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Good CD25 PE/Cy7 Antibody for Flow Cytometry
(1)
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BioLegend
June 15, 2023
We use this antibody to look at regulatory T cell phenotype from the mouse lymph nodes in various genetic and other perturbation models of ...
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Efficient Proteasome Inhibitor for Studying Cell Death
(1)
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Selleck Chemicals
June 15, 2023
In our lab, we conduct many experiments studying cell death induction. For that, we also use Bortezomib from Sellekchem and are more than ...
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Great I-A/I-E Antibody with Strong Staining
(1)
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BioLegend
June 15, 2023
We use this antibody often to identify and characterize different antigen presenting cells from the mouse spleen and lymph nodes. It stains ...
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Very Clean Cis-Golgi Marker
(1)
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BD Biosciences
June 15, 2023
My research involves studying how D1 dopamine receptor, a member of the GPCR superfamily, can signal from distinct membrane compartments ...
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Conjugated SNAP-Antibody Suitable for Live Cell Imaging
(1)
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New England Biolabs
June 15, 2023
This research involves studying how D1 dopamine receptor, a member of the GPCR superfamily, can signal from distinct membrane compartments ...
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Adaptable AF-488 Secondary Antibody for Immunofluorescence
(1)
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Abcam
June 15, 2023
Human embryonic stem cells were differentiated to epicardial cells and then stimulated with one of 4 different growth factors to promote ...
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Antibody Stains MHC II Very Well
(1)
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June 14, 2023
We wanted to assess how certain perturbations would affect the activation of immune cells in the spleen, so we used the antibody to identify...
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Good CD90.1 PerCP Antibody for Flow Cytometry
(1)
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BioLegend
June 14, 2023
This antibody is crucial for us to stain for congenic T cells that we have adoptive transferred into mice. We can distinguish between ...
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Excellent TUBB3 Antibody for Immunofluorescence
(1)
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BioLegend
June 14, 2023
Very good for immunofluorescence staining of mouse enteric neurons. High specificity with low background.
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Good CD62L BV570 Antibody from BioLegend
(1)
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BioLegend
June 14, 2023
We usually use this antibody to detect naive and effector T cells in mouse spleen and lymph nodes. We can clearly define different ...
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Good D1 Dopamine Receptor Antibody
(1)
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Abcam
June 14, 2023
My research involves studying how D1 dopamine receptor, a member of the GPCR superfamily, can signal from distinct membrane compartments ...
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Good SLC22A2 Antibody for Organic Cation Transporter 2
(1)
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Abcam
June 14, 2023
My research involves studying how D1 dopamine receptor, a member of the GPCR superfamily, can signal from distinct membrane compartments ...
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Great CCR7 BV421 Antibody from BioLegend
(1)
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BioLegend
June 13, 2023
We use this antibody for flow cytometry to look at different populations of dendritic cells from the mouse lymph nodes. This antibody works ...
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Good SIRPa Antibody for Flow Cytometry
(1)
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BioLegend
June 13, 2023
We use this antibody to stain for different subsets of dendritic cells from mouse lymphoid tissues in order to phenotype their activation ...
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Stable Substrate for In Vivo Imaging Using IVIS
(1)
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Promega
June 13, 2023
We used this kit to tracking luciferase activity in a time-dependent manner in vivo. The kit gave us clear and stable results through our ...
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Easy to Use Cytotoxicity Assay
(1)
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Promega
June 13, 2023
We used this kit to measure the cell viability in response to various treatment. We used many different types of cell, from tumor cell lines...
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Excellent CD20 Antibody for Immunohistochemistry
(1)
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June 13, 2023
This antibody is excellent for mammalian species on formol fixed paraffin embedded tissue Immunohistochemical stains.
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Great CD38 Antibody from BioLegend
(1)
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BioLegend
June 13, 2023
We are an immunotherapy focused group with an interest in CD8 T-cells and their TCRs. For our experimental purposes, we routinely isolate ...
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Easy to Use Kit for RNA Extraction
(1)
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Favorgen Biotech Corp.
June 12, 2023
The kit is used to extract RNA from a wide variety of samples such as animal tissue; cultured cells of animal, fungus or bacteria. It is ...
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Great Anti-Succinyllysine Antibody for Western Blotting
(1)
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June 12, 2023
Great antibody for detecting overall succinyllysine levels in MCF10a cells. A lot of these antibodies used to detect overall levels of post ...
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Good Buffer to Lyse Cells for Protein Detection
(1)
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Thermo Fisher Scientific
June 12, 2023
It is ready-to-use solution for getting extraction of cells, does not need to assemble, and is very fast.
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Good ABCA1 Antibody from Abcam
(1)
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June 12, 2023
Experiment did to test the effect of 25-OH cholesterol on ABCA1 expression in human THP-1 cell. The results showed 25-OH cholesterol did ...
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